Publications related to Coherent Imaging of Cellular Dynamics

Diffraction-unlimited all-optical imaging and writing with a photochromic GFP

Lens-based optical microscopy failed to discern fluorescent features closer than 200 nm for decades, but the recent breaking of the diffraction resolution barrier by sequentially switching the fluorescence capability of adjacent features on and off is maki ...

2011

Direct Investigation of Intracellular Presence of Gold Nanoparticles

Francesco Stellacci, Randy Patrick Carney

Nanotechnology as well as advanced microscopy can play a fundamental role in understanding biological mechanisms. Here we present a study that combines a new type of nanomaterial with a new type of microscopy and highlights the potential for gathering nove ...

2011

TubeSpin bioreactor 50 for the high-density cultivation of Sf-9 insect cells in suspension

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Xiaowei Zhang, Qiuling Xie

Here we present the TubeSpin bioreactor 50 (TubeSpins) as a simple and disposable culture system for Sf-9 insect cells in suspension. Sf-9 cells had substantially better growth in TubeSpins than in spinner flasks. After inoculation with 106 cells/ml, maxim ...

Springer Netherlands2011

Transient State Monitoring by Total Internal Reflection Fluorescence Microscopy

Theo Lasser, Thiemo Spielmann

Triplet, photo-oxidized and other photoinduced, long-lived states of fluorophores are sensitive to the local environment and thus attractive for microenvironmental imaging purposes. In this work, we introduce an approach where these states are monitored in ...

2010

Three-dimensional nano-localization of single fluorescent emitters

Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler, Noelia Laura Bocchio

We present a combination of self-interference microscopy with lateral super-resolution microscopy and introduce a novel approach for localizing a single nano-emitter to within a few nanometers in all three dimensions over a large axial range. We demonstrat ...

Optical Society of America2010

A Versatile Route to Red-Emitting Carbopyronine Dyes for Optical Microscopy and Nanoscopy

Marcel Leutenegger

Biological microscopy favors photostable fluorescent markers with large fluorescence quantum yields, low dark triplet state population, good biocompatibility and absorption and emission maxima in the near-infrared, where cellular autofluorescence is minimi ...

2010

Characterization of the cytotoxicity and imaging properties of secondharmonic nanoparticles

Demetri Psaltis, Ye Pu, Rachel Grange, Chia-Lung Hsieh

We develop second-harmonic nanoparticles as the contrast agents for cell imaging. Second-harmonic nanoparticles show promise as cell imaging probes due to their non-bleaching, non-blinking, and coherent signal. Nanoparticles of noncentrosymmetric crystal s ...

Spie-Int Soc Optical Engineering, Po Box 10, Bellingham, Wa 98227-0010 Usa2010

Photoactivatable Probes for Protein Labeling

Sambashiva Banala

Over the last decade, several protein tags have been developed for the specific and covalent labeling of fusion proteins with small organic probes. Among them, SNAP-tag has been used in many applications such as live cell imaging, protein microarrays and t ...

EPFL2010

Microfluidic devices made of UV-curable glue (NOA81) for fluorescence detection based applications

Nico de Rooij, Philip Wägli, Blaise Guélat

The UV-curable adhesive NOA81 (Norland Optical Adhesive) is a promising liquid photopolymer for low-cost microfluidic chip production. Different fabrication methods for NOA81 are suggested [1,2]. However, optical characterization for fluorescent based dete ...

2010

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

Michaël Unser, Matthieu Guerquin-Kern, Ilker Bayram, Raquel Terrés Cristofani

Modern deconvolution algorithms are often specified as minimization problems involving a non-quadratic regularization functional. When the latter is a wavelet-domain

ℓ _{ 1 }

-norm that favors sparse solutions, the problem can be solved by a simple it ...

IEEE2010

Coherent Imaging of Cellular Dynamics

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Diffraction-unlimited all-optical imaging and writing with a photochromic GFP

Direct Investigation of Intracellular Presence of Gold Nanoparticles

TubeSpin bioreactor 50 for the high-density cultivation of Sf-9 insect cells in suspension

Transient State Monitoring by Total Internal Reflection Fluorescence Microscopy

Three-dimensional nano-localization of single fluorescent emitters

A Versatile Route to Red-Emitting Carbopyronine Dyes for Optical Microscopy and Nanoscopy

Characterization of the cytotoxicity and imaging properties of secondharmonic nanoparticles

Photoactivatable Probes for Protein Labeling

Microfluidic devices made of UV-curable glue (NOA81) for fluorescence detection based applications

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

Characterization of the cytotoxicity and imaging properties of secondharmonic nanoparticles

Transient State Monitoring by Total Internal Reflection Fluorescence Microscopy

Photoactivatable Probes for Protein Labeling

Direct Investigation of Intracellular Presence of Gold Nanoparticles

Diffraction-unlimited all-optical imaging and writing with a photochromic GFP

A Versatile Route to Red-Emitting Carbopyronine Dyes for Optical Microscopy and Nanoscopy

Microfluidic devices made of UV-curable glue (NOA81) for fluorescence detection based applications

TubeSpin bioreactor 50 for the high-density cultivation of Sf-9 insect cells in suspension

Three-dimensional nano-localization of single fluorescent emitters

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy