Global and local anthropogenic effects on hydrodynamics of lakes

In many countries water quality is a major concern for drinking water management authorities. Freshwater is a limited resource and the demand for good-quality water from natural aquatic systems is therefore high. Additional stress arises from anthropogenic emissions of nutrients, chemicals, pathogens, heat and hydrological alterations as well as from the ongoing global climate change. In this thesis the relevant recent past, present and future factors affecting water quality in the perialpine Lake Biel, used as a source for drinking water, was investigated. Through measurements in the field combined with hydrodynamic logical modelling in one and three dimensions, optimal locations for forthcoming lake water intakes was identified. Water quality properties considered were temperature, oxygen, suspended sediment concentration (SSC) and the risk of subaqueous mass movements. The results identified the forthcoming changes expected in Lake Biel due to the upcoming decommission of the Mühleberg Nuclear Power Plant (MNPP) in 2019. This plant currently release ~700 MW of heat into the upstream Aare River in the form of cooling water. The removal of this anthropogenic thermal source will decrease future lake temperature by ~0.3 °C (volume average). Due to seasonal river discharge patterns, the main impact of the plant closure will occur during winter. Minor effects are expected in summer in the form of moderately weaker lake stratification. Furthermore, the current fate of thermal pollution emitted from MNPP was linked to the hydraulic residence time of Lake Biel. While short-term anthropogenic thermal impacts in Lake Biel can be large, the system is additionally experiencing continuous warming up to ~0.1 °C per decade (volume average) by the ongoing climate change. The climate effect on Lake Biel and its primary tributary, the Aare River, was investigated as well as compared to the much larger Lake Geneva and Rhône River. Climate change causes seasonal river discharge shifts resulting in enhanced river warming in summer and diminished warming in winter, while at the same time SSC increases in winter and decreases in summer. Differences in temperature as well as warming rates between rivers and lakes in turn resulted in a discharge and hydraulic residence time-dependent decrease in climate warming of lakes. Furthermore, deep-water renewal in both lakes is predicted to increase in summer and decreases in winter, possibly influencing the replenishment of deep water oxygen. Additionally, sedimentation patterns in Lake Biel were examined and linked to the historical diversion of the Aare River into the lake. The majority of sediment supplied to Lake Biel (~80 %) came from large SSC events in the Aare. These events resulted in a selective particle settling pattern, which concentrated sedimentation on the shallow shelf area North-East of the Aare delta. Both the large SSC events as well as this selective sedimentation pattern were connected to weather fronts coming in from the Atlantic Ocean. The research performed here opens up for further promising interdisciplinary research between Meteorology, Climatology, Hydrology and Sedimentology. Inland water management would benefit through increased knowledge regarding the propagation of particles and/or anthropogenic heat, from river catchments into downstream lakes and sediments.

Sources, Spreading and Fate of Antibiotic Resistance Genes and Resistant Bacteria in Vidy Bay, Lake Geneva, Switzerland

Nadine Czekalski

Increasing numbers of multiresistant bacterial pathogens cause severe health problems on a global scale. Large quantities of bacteria and their resistance genes are discharged into natural environments via human and animal waste. Not much is known so far about the fate and further spread of resistance determinants in the environment. There is evidence that aquatic environments may function as important compartments for preserving and exchanging antibiotic resistance genes (ARG) from sewage and drinking water. Lake Geneva is the largest fresh water reservoir of Western Europe and supplies the surrounding cities. At the same time these cities discharge sewage into the lake. Lausanne is the largest city in the lake’s catchment and discharges the highest amount of treated, as well as in case of heavy rain events, partly untreated waste waters. Also two rivers, Chambering and Flown, transport untreated sewage (bypass), in case of storm events. Only 3.4 km from the sewage discharge point lake water is pumped for drinking water preparation for Lausanne. Many studies have reported on contamination of the bay with heavy metals, inorganic nutrients, organic carbon and fecal indicator bacteria due to the sewage discharge. The present thesis aimed on investigating the sources, spreading and fate of antibiotic resistant bacteria and antibiotic resistance genes in the Vida bay, Lake Geneva. In the first part of this dissertation, a screening for antibiotic- and specifically multi-resistant bacteria was carried out as one of the first studies in a Swiss waste water stream and the receiving fresh water lake. For this purpose, a combined set of culture based (viable bacterial counts on nutrient media supplemented with antibiotics) and molecular tools (detection and quantitation of antibiotic resistance genes from environmental DNA-extracts using quantitative real-time PCR) was applied. Raw hospital and communal sewage, as well as partly treated/mixed sewage, which is discharged into the lake via an effluent pipe 700 m off shore, were taken into account. Furthermore lake water samples nearby the end of the waste water discharge pipe and the drinking water pump as well as close to the two river mouths were analyzed. Finally, the role of Lausannes WTP was evaluated for its potential to remove resistant and multiresistant bacteria, as well ARG. Results show highest levels of multiresistant bacteria and ARG in both hospital and communal sewage. Incidence was found that the WTP is a selective environment for multiresistant bacteria and resistance genes. Moreover, lake water and sediments nearby the end of the WTP discharge pipe and the two river mouths exhibited significantly higher levels of resistant determinants as compared to samples from the drinking water uptake. If the level of antibiotic resistance close to the drinking water uptake represents the natural background level of the lake or whether it is impacted by the WTP plume remained open during this first survey. The spatial distribution and persistence of ARG discharged via waste water into lake systems has been evaluated for the first time in the second part of this thesis. 22 sediment cores were sampled along two major transects (leading either into the deep lake or along the south-western shore line to the drinking water uptake) and in the vicinity of the WTP discharge pipe in the Vidy bay. A subset of samples was also analyzed for contamination with a “conventional” pollutant of sewage (mercury), for which a similar transport behavior (particle bound) as for bacteria and their resistance genes is assumed. The microbial community composition in all sediment as well as in waste water samples was analyzed using ARISA. Strong pollution with both ARG and mercury was detected in close proximity of the WTP discharge pipe and both contaminants significantly decreased along the transect into the deep lake. In general however, the spatial contamination of mercury and ARG was only moderately correlated. While the mercury contaminated sediment surface area tended to have a pollution tail in south eastern direction, ARG showed only a slow decrease along the south western transect. These findings imply differences in transport and fate of mercury and ARG in the bay, or additional sources of ARG contamination. Analysis of the microbial diversity in the samples support these hypotheses. This study revealed feasibility of qPCR as a tool to semi-quantitatively track spatial ARG contamination in fresh water lake sediments. Not much is known yet on the antibiotic resistance background level of fresh water lakes and how this may be influenced by anthropogenic activities in the surrounding catchment. Water samples from 21 Swiss lakes have been collected and were analyzed for the prevalence of different ARG (2 sulfonamide resistance genes sul1 & sul2, 3 tetracycline resistance genes tetB, tetM, tetW and one plasmid mediated fluoroquinolone resistance gene qnrA). In order to document the abundance of these genes in the bacterial populations, they were normalized to bacterial 16S rRNA gene copy numbers. The lakes differ in size, geographical position, land use pattern, and trophical status. The composition of the microbial community of all lakes was analyzed using ARISA. Sulfonamide resistance genes were detected in all investigated lakes (0.2-4%, sul1 and 0.01-0.3%, sul2), with highest levels in Lake Baldegger (21%). Lake Baldegger also contained all of the three tetracycline resistance genes, which were found in only 4 other lakes. None of the lakes was positive for qnrA. Important anthropogenic catchment activities linked to sul gene prevalence could not be identified based on our data and may require further analysis and an additional variables to complete our data set. However, our study demonstrates contamination of Swiss lakes with sulfonamide resistance genes and the potential of fresh water lakes preserve these genes, even remote from direct sources of pollution.

EPFL2013

Effects of climate change on deep-water oxygen and winter mixing in a deep lake (Lake Geneva)

Damien Bouffard, Robert Vincent Schwefel, Alfred Johny Wüest

Oxygen is the most important dissolved gas for lake ecosystems. Because low oxygen concentrations are an ongoing problem in many parts of the oceans and numerous lakes, oxygen depletion processes have been intensively studied over the last decades and were mainly attributed to high nutrient loads. Recently, climate-induced changes in stratification and mixing behavior were recognized as additional thread to hypolimnetic oxygen budgets in lakes and reservoirs [Matzinger et al., 2007; Zhang et al., 2015]. Observational data of Lake Geneva, a deep perialpine lake situated between France and Switzerland showed no decreasing trend in hypoxia over the last 43 years, despite an impressive reduction in nutrient input during this period. Instead, hypoxic conditions were predominantly controlled by deep mixing end of winter and in turn by winter temperatures. To test the sensitivity of Lake Geneva on future climate change and changes in water transparency, we simulated the hydrodynamics and temperature of Lake Geneva under varying conditions for atmospheric temperature and water clarity performed with the one-dimensional model SIMSTRAT [Goudsmit, 2002]. The results show, that the stratification in lakes is only weakly affected by changes in light absorption due to varying water quality. For conditions expected for the end of the century, a decrease in the annual mean deep convective mixing of up to 45 m is predicted. Also complete mixing events over the whole lake are less likely to occur. A change in the hypolimnetic oxygen concentration of up to 20% can thus be expected in the future. These results show, that changes in deep mixing have an equally strong impact as eutrophication on the deep-water oxygen development of oligomictic lakes and have to be considered in the prediction of the future development of lakes.

2016

Oxygen depletion in stratified lakes - why shallow and deep lakes react differently

Robert Vincent Schwefel, Alfred Johny Wüest

Oxygen is the most important element in natural waters for any higher aquatic life, such as fish. As with humans, almost all members of aquatic ecosystems need oxygen to breathe. Building on this, treatment of drinking water is easier for oxic than for anoxic water. Critical for hypoxic conditions in lakes are the deep waters during stagnation periods, typically during summer or warm/wet periods, when the water columns are density stratified. Then, the oxygen supply to the deep water is restrained and the decomposition of organic matter leads to oxygen depletion in the stratified deep layers. Especially in shallow lakes, the resulting oxygen removal can be drastic. In this presentation, we show how oxygen depletion mechanisms in lakes take place and how depletion is related to key processes such as primary productivity (via nutrient loading), molecular diffusion of oxygen into the sediment, molecular diffusion of reduced substances out of the sediment and, finally, the deep convective mixing in winter which refills the oxygen reservoirs of the deep layers. Here, we will clarify how these different processes affect the oxygen budget: primary production drives oxygen depletion directly, whereas lake morphology and external wind forcing influence the rate of depletion. Finally, climate change can affect the replenishment of oxygen in the deep water and thereby change the oxygen budget. The examples shown in this presentation will be set into the context of the water quality in Switzerland, which has gone through a rigorous improvement in the last 40 years.

2016

Sources, Spreading and Fate of Antibiotic Resistance Genes and Resistant Bacteria in Vidy Bay, Lake Geneva, Switzerland

Nadine Czekalski

EPFL2013