Total internal reflection fluorescence microscopeA total internal reflection fluorescence microscope (TIRFM) is a type of microscope with which a thin region of a specimen, usually less than 200 nanometers can be observed. TIRFM is an imaging modality which uses the excitation of fluorescent cells in a thin optical specimen section that is supported on a glass slide. The technique is based on the principle that when excitation light is totally internally reflected in a transparent solid coverglass at its interface with a liquid medium, an electromagnetic field, also known as an evanescent wave, is generated at the solid-liquid interface with the same frequency as the excitation light.
Depth of fieldThe depth of field (DOF) is the distance between the nearest and the furthest objects that are in acceptably sharp focus in an image captured with a camera. For cameras that can only focus on one object distance at a time, depth of field is the distance between the nearest and the farthest objects that are in acceptably sharp focus. "Acceptably sharp focus" is defined using a property called the "circle of confusion". The depth of field can be determined by focal length, distance to subject, the acceptable circle of confusion size, and aperture.
Canon Inc.Canon Inc. (キヤノン株式会社; Hepburn: hepburn) is a Japanese multinational corporation headquartered in Ōta, Tokyo, specializing in optical, imaging, and industrial products, such as lenses, cameras, medical equipment, , printers, and semiconductor manufacturing equipment. Canon has a primary listing on the Tokyo Stock Exchange and is a constituent of the TOPIX Core 30 and Nikkei 225 indexes. It used to have a secondary listing on the New York Stock Exchange. The company was originally named hepburn (精機光学研究所).
