Publications related to Photons in - numbers out: perspectives in quantitative fluorescence microscopy for in situ protein counting

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

Michaël Unser, Matthieu Guerquin-Kern, Ilker Bayram, Raquel Terrés Cristofani

Modern deconvolution algorithms are often specified as minimization problems involving a non-quadratic regularization functional. When the latter is a wavelet-domain

ℓ _{ 1 }

-norm that favors sparse solutions, the problem can be solved by a simple it ...

IEEE2010

Super-resolution fluorescence microscopy based on physical models

François Aguet

This thesis introduces a collection of physics-based methods for super-resolution in optical microscopy. The core of these methods constitutes a framework for 3-D localization of single fluorescent molecules. Localization is formulated as a parameter estim ...

EPFL2009

Single Molecule Interference: Towards Three-Dimensional Fluorescence Localization Microscopy at the Molecular Level

Theo Lasser, Iwan Märki

We demonstrate three-dimensional, nanometer-scale positioning precision of a single quantum-dot using single molecule interference and localization. We anticipate that our approach will lay the foundation for fluorescence microscopy with molecular resoluti ...

2009

An accurate PSF model with few parameters for axially shift-variant deconvolution

Michaël Unser, Dimitri Nestor Alice Van De Ville, François Aguet

Accurate knowledge of an imaging system's point spread function (PSF) is crucial for successful deconvolution. For fluorescence microscopy, PSF estimations based on either theoretical models or experimental measurements are available. However, due to the a ...

Ieee Service Center, 445 Hoes Lane, Po Box 1331, Piscataway, Nj 08855-1331 Usa2008

An Accurate PSF Model with Few Parameters for Axially Shift-Variant Deconvolution

Michaël Unser, Dimitri Nestor Alice Van De Ville, François Aguet

Accurate knowledge of an imaging system's point spread function (PSF) is crucial for successful deconvolution. For fluorescence microscopy, PSF estimations based on either theoretical models or experimental measurements are available. However, due to the ...

IEEE2008

Detection efficiency in total internal reflection fluorescence microscopy

Theo Lasser, Marcel Leutenegger

We present a rapid and flexible framework for the accurate calculation of the detection efficiency of fluorescence emission in isotropic media as well as in the vicinity of dielectric or metallic interfaces. The framework accounts for the dipole characteri ...

2008

Repetitive Reversible Labeling of Proteins at Polyhistidine Sequences for Single-Molecule Imaging in Live Cells

Horst Vogel, Rudolf Hovius, Jean-Manuel Segura, Emmanuel Guignet

Sensitive live-cell fluorescence microscopy and single-molecule imaging are severely limited by rapid photobleaching of fluorescent probes. Herein, we show how to circumvent this problem using a novel, generic labeling strategy. Small nickel-nitrilotriacet ...

2007

Single molecule detection on surfaces

Marcel Leutenegger

Monitoring biological relevant reactions on the single molecule level based on fluorescence spectroscopy techniques has become one of the most promising approaches for understanding a variety of phenomena in biophysics, biochemistry and life science. By ap ...

EPFL2007

The Colored Revolution of Bioimaging

Michaël Unser, Cédric René Jean Vonesch, François Aguet

With the recent development of fluorescent probes and new high-resolution microscopes, biological imaging has entered a new era and is presently having a profound impact on the way research is being conducted in the life sciences. Biologists have come to d ...

IEEE2006

Processive movement of single kinesins on crowded microtubules visualized using quantum dots

Arne Seitz

Kinesin-1 is a processive molecular motor transporting cargo along microtubules. Inside cells, several motors and microtubule-associated proteins compete for binding to microtubules. Therefore, the question arises how processive movement of kinesin-1 is af ...

Nature Publishing Group2006

Photons in - numbers out: perspectives in quantitative fluorescence microscopy for in situ protein counting

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Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

Super-resolution fluorescence microscopy based on physical models

Single Molecule Interference: Towards Three-Dimensional Fluorescence Localization Microscopy at the Molecular Level

An accurate PSF model with few parameters for axially shift-variant deconvolution

An Accurate PSF Model with Few Parameters for Axially Shift-Variant Deconvolution

Detection efficiency in total internal reflection fluorescence microscopy

Repetitive Reversible Labeling of Proteins at Polyhistidine Sequences for Single-Molecule Imaging in Live Cells

Single molecule detection on surfaces

The Colored Revolution of Bioimaging

Processive movement of single kinesins on crowded microtubules visualized using quantum dots

Single molecule detection on surfaces

The Colored Revolution of Bioimaging

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

Super-resolution fluorescence microscopy based on physical models

Single Molecule Interference: Towards Three-Dimensional Fluorescence Localization Microscopy at the Molecular Level

An accurate PSF model with few parameters for axially shift-variant deconvolution

Detection efficiency in total internal reflection fluorescence microscopy

Processive movement of single kinesins on crowded microtubules visualized using quantum dots

An Accurate PSF Model with Few Parameters for Axially Shift-Variant Deconvolution

Repetitive Reversible Labeling of Proteins at Polyhistidine Sequences for Single-Molecule Imaging in Live Cells