Allosteric Protein Switches based on Antibody Mimetics
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A chimeric TASP (Template Assembled Synthetic Proteins) mol., consisting of a 4-helix bundle motif and an antibody recognition site, has been covalently attached to a gold surface. The reversible antibody binding to the self-assembled monolayer as monitore ...
Characterizing the movement, interactions, and chem. microenvironment of a protein inside the living cell is crucial to a detailed understanding of its function. Most strategies aimed at realizing this objective are based on genetically fusing the protein ...
O6-substituted guanine derivs. are described for use as substrates for O6-alkylguanine-DNA alkyltransferases (AGT) that can be used to label the enzyme or fusion proteins contg. it. Proteins of interest are incorporated into an AGT fusion protein, the AGT ...
(Ecole Polytechnique Federale de Lausanne (EPFL), Switz.).2004
The present invention relates to methods of transferring a label from novel substrates to O-alkylguanine-DNA alkyltransferases (AGT) and O-alkylguanine-DNA alkyltransferase fusion proteins, and to novel substrates suitable in such methods. Proteins o ...
An experimental system is described, permitting a detailed and systematic analysis of the factors governing self-assembly of amphipathic helices, e.g. to a four-helical bundle, a subject of major relevance for tertiary structure formation. protein folding ...
A symposium report. The concept of template assembled synthetic proteins (TASP) has been evaluated for the locked-in fold derived from the DNA-binding bba zinc finger motif based on the consensus sequence Xaa3-Cys-Xaa2-4-Cys-Xaa12-His-Xaa3-4-His-Xaa4 to mi ...
We report here the generation of mutants of the human O6-alkylguanine-DNA alkyltransferase (hAGT) for the efficient in vivo labeling of fusion proteins with synthetic reporter mols. Libraries of hAGT were displayed on phage, and mutants capable of efficien ...
A method using O6-alkylguanine-DNA alkyltransferases (AGT) is disclosed for transferring a label from a substrate to a fusion protein comprising the AGT. This allows the detection and/or manipulating of the fusion protein, both in vitro and in vivo, by att ...
(Ecole Polytechnique Federale De Lausanne, Switz.).2002
The construction of protein-like folding motifs as structurally stable scaffolds for the introduction of function represents a major goal in protein design. The use of topol. templates allows the bypass of the well-known folding problem of linear polypepti ...
A symposium report on synthetic strategies which provide a synthetic entry to a new generation of template assembled synthetic proteins (TASP). [on SciFinder (R)] ...
