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Waveguide platform and methods for super-resolution fluorescence microscopy of sub-cellular structures

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Surface passivation and functionalisation for mass photometry

Suliana Manley, Jenny Sülzle, Laila Abdelaziz Abdelmoniem Elfeky

Interferometric scattering (iSCAT) microscopy enables the label-free observation of biomolecules. Consequently, single-particle imaging and tracking with the iSCAT-based method known as mass photometry (MP) is a growing area of study. However, establishing ...

Wiley2024

Fluorescence microscopy: A statistics-optics perspective

Aleksandra Radenovic

Fundamental properties of light unavoidably impose features on images collected using fluorescence microscopes. Accounting for these features is often critical in quantitatively interpreting microscopy images, especially those gathering information at scal ...

Amer Physical Soc2024

The multimodality cell segmentation challenge: toward universal solutions

Sahand Jamal Rahi, Vojislav Gligorovski, Marco Labagnara, Jun Ma, Xin Yang, Maxime Emmanuel Scheder, Yao Zhang, Bo Wang, Yixin Wang, Lin Han

Cell segmentation is a critical step for quantitative single-cell analysis in microscopy images. Existing cell segmentation methods are often tailored to specific modalities or require manual interventions to specify hyper-parameters in different experimen ...

Nature Portfolio2024

Practical computational imaging by use of spatiotemporal light modulation: from simulations to applications in biological microscopy

François Thierry M Marelli

Optical microscopy is an essential tool for biologists, who are often faced with the need to overcome the spatial and temporal resolution limitations of their devices to capture finer details. As upgrading imaging hardware is expensive, computational metho ...

EPFL2023

Advancing atomic force microscopy through open source instrumentation

Mustafa Kangül

Atomic force microscopy (AFM), a member of the scanning probe microscopy (SPM) family, holds a unique position as a nano-characterization instrument in the fields of physics, chemistry, and biology. Its ability to provide atomic resolution and operate in v ...

EPFL2023

Chemical Probe for Imaging of Polo-like Kinase 4 and Centrioles

Kai Johnsson, Luc Reymond, Georgios Hatzopoulos, Aleksandar Salim

Polo-like kinase (Plk4) is a serine/threonine-proteinkinase thatis essential for biogenesis of the centriole organelle and is enrichedat centrioles. Herein, we introduce Cen-TCO, a chemical probe basedon the Plk4 inhibitor centrinone, to image Plk4 and cen ...

AMER CHEMICAL SOC2023

Event-driven acquisition for content-enriched microscopy

Suliana Manley, Martin Weigert, Chen Zhang, Willi Leopold Stepp, Dora Mahecic, Juliette Griffie

Event-driven acquisition uses neural-network-based recognition of specific biological events to trigger switching between slow and fast super-resolution imaging, enriching the capture of interesting events with high spatiotemporal resolution. A common goal ...

NATURE PORTFOLIO2022

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Single-molecule localization microscopy (SMLM) can reveal nanometric details of biological samples, but its high phototoxicity hampers long-term imaging in live specimens. A significant part of this phototoxicity stems from repeated irradiations that are n ...

WILEY-V C H VERLAG GMBH2022

Fluorescence lifetime imaging microscopy (FLIM) is an imaging modality often used to monitor biochemical properties of a cell or a tissue. In addition to conventional fluorescence microscopy features, such as selective labeling and non-invasiveness, FLIM e ...

EPFL2021

Rhodamines are the most important class of fluorophores for applications in live-cell fluorescence microscopy. This is mainly because rhodamines exist in a dynamic equilibrium between a fluorescent zwitterion and a nonfluorescent but cell-permeable spirocy ...

AMER CHEMICAL SOC2021