Two-photon imaging and selective laser ablation of Cochlea hair cells through a multimode fiber probe

Eirini Kakkava, Damien Claude-Marie Loterie, Christophe Moser, Demetri Psaltis, Marilisa Romito
SPIE-INT SOC OPTICAL ENGINEERING, 2019
Conference paper

We demonstrate multimode fiber probe that accomodates dual modality properties for high power ultrashort pulse delivery. A commercially available multimode graded-index (GRIN) fiber is used for two-photon imaging and/or femtosecond laser ablation of Cochlea hair cells. Lensless focusing and digital scanning of ultrashort pulses through the optical fiber is realized using the transmission matrix technique. We investigate the performance and the limitations of the GRIN probe in terms of focusing efficiency and peak power delivery. Selective laser ablation guided by the twophoton image obtained through the GRIN fiber is realized by proximally-only control of the femtosecond laser beam.

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Eirini Kakkava, Damien Claude-Marie Loterie, Christophe Moser, Demetri Psaltis, Marilisa Romito
SPIE-INT SOC OPTICAL ENGINEERING, 2019
Conference paper

Abstract

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https://infoscience.epfl.ch/record/266726?ln=en

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Wavefront shaping for ultrashort pulse delivery through optical fibers for imaging and ablation

Donald Benjaman Conkey, Eirini Kakkava, Damien Claude-Marie Loterie, Christophe Moser, Demetri Psaltis, Nicolino Stasio

We demonstrate high power ultrashort pulse delivery through a commercially available multicore fiber (MCF) and a multimode graded-index fiber (GRINF) for imaging and laser ablation. Lensless focusing and digital scanning of ultrashort pulses through the optical fibers is realized using wavefront shaping. We compare the performance of the two systems in terms of focusing efficiency and peak power delivery. Furthermore, we investigate the limitations that nonlinearities induce when high peak power ultrashort pulses are launched in MCFs and GRIN fibers. Proximally-only controlled two-photon fluorescence imaging and laser ablation are demonstrated through both investigated systems.

SPIE-INT SOC OPTICAL ENGINEERING2018

Selective femtosecond laser ablation via two-photon fluorescence imaging through a multimode fiber

Donald Benjaman Conkey, Eirini Kakkava, Damien Claude-Marie Loterie, Christophe Moser, Demetri Psaltis, Marilisa Romito

We demonstrate the ability of a multimode fiber probe to provide two-photon fluorescence (TPF) imaging feedback that guides the femtosecond laser ablation (FLA) in biological samples for highly selective modifications. We implement the system through the propagation of high power femtosecond pulses through a graded-index (GRIN) multimode fiber and we investigate the limitations posed by the high laser peak intensities required for laser ablation. We demonstrate that the GRIN fiber probe can deliver laser intensities up to 1.5x10(13) W/cm(2). sufficient for the ablation of a wide range of materials, including biological samples. Wavefront shaping through an ultrathin probe of around 400 mu m in diameter is used for diffraction limited focusing and digital scanning of the focus spot. Selective FLA of cochlear hair cells is performed based on the TPF images obtained through the same multimode fiber probe. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

OPTICAL SOC AMER2019

Two-photon fluorescence imaging through multicore fiber with digital phase conjugation

Donald Benjaman Conkey, Christophe Moser, Demetri Psaltis, Nicolino Stasio

We present near diffraction limited two photon fluorescence (TPF) imaging through a lensless, multi-core fiber (MCF) endoscope utilizing digital phase conjugation. The ultra-small size of MCFs make them desirable tools for imaging deep into the body. TPF imaging enables optical sectioning and is widely used in brain and biological imaging and is a desired modality for fiber endoscopes. Previous implementations of TPF imaging through MCFs focus and scan the light from individual cores for image formation. In such systems the resolution is limited by the MCF core spacing, although a lens may be used to improve the resolution at the expense of the field of view. Other, more recent work has improved the resolution limitation using custom built MCFs for focusing and scanning of ultrafast pulses using wavefront shaping. Here we present digital phase conjugation for ultrafast pulse focusing through a MCF for an imaging resolution independent of the MCF core spacing. Furthermore, the phase conjugation technique does not require the use of a lens at the fiber end for focus formation and is compatible with commercially available MCFs with a large number of cores. Here, we present a 3000 core MCF endoscope and demonstrate ultrafast pulse focusing with sufficient focus spot contrast and power for TPF endoscope imaging. We construct TPF images by digital scanning of the phase conjugated focus on the target object and collection of the emitted fluorescence through the MCF. This work demonstrates the viability of digital conjugation combined with commercially available MCFs for higher resolution lensless, two photon endoscopy.

Spie-Int Soc Optical Engineering2016

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