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Characterization of Coenzyme Q Biosynthesis Proteins through Integrative Modeling at the Protein-Membrane Interface

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Cadmium selenide nanocrystals for specific interactions with biomolecules

The goal of this work was to produce water-soluble fluorescent semiconductor nanocrystals suitable to interact specifically with biomolecules and thereby creating hybrid supramolecular structures composed of nanocrystals and proteins. Hydrophobic TOPO-coat ...

EPFL2005

Structural basis for lipid-mediated interactions between mitochondrial ADP/ATP carrier monomers

The oligomerization state of the ADP/ATP carrier is an important issue in understanding the mechanism underlying nucleotide exchange across the inner mitochondrial membrane. The first high resolution structure obtained in the presence of carboxyatractylosi ...

Elsevier2005

Oiling the key hole

Françoise Gisou van der Goot Grunberg

Many bacteria have been found to interact with specialized domains, rich in cholesterol and sphingolipids, of the host plasma membrane, termed lipid rafts. The mechanisms that underlie this interaction are starting to be unravelled. In this issue, Hayward ...

2005

Pumping of mammalian cells with a nozzle-diffuser micropump

Martinus Gijs, Horst Vogel, Caroline Vandevyver, Christophe Yamahata, Frédéric Lacharme, Paulina Izewska

We discuss the successful transport of jurkat cells and 5D10 hybridoma cells using a reciprocating micropump with nozzle-diffuser elements. The effect of the pumping action on cell viability and proliferation, as well as on the damaging of cellular membran ...

Royal Society of Chemistry2005

Native membranes on subwavelentgh holes for membrane receptors analysis

Jürgen Brugger, Benjamin André René Meyer

How membrane receptors transduce external signals across the membrane and activate intracellular processes is a key question for fundamental research and for therapeutic purposes. Reliable in situ investigations requiest to preserve the full biological act ...

2004

Two-phase partition profiling of drugs and ionisable compounds

Lipophilicity of compounds with therapeutic interest represents one of the most important physicochemical parameters in predicting and interpreting transport processes across biological barriers. This property is commonly measured as a drug | membrane part ...

EPFL2004

Permeation liquid membrane as a tool for monitoring bioavailable Pb in natural waters

In order to predict metal bioavailability by microorganisms in natural waters, analytical speciation techniques such as the permeation liquid membrane (PLM) are required. A planar sheet PLM has been characterized by measuring Pb fluxes in the absence and p ...

2004

Importin alpha associates with membranes and participates in nuclear envelope assembly in vitro

Importin alpha is well known as an adaptor that functions with Importin beta in the nuclear import of proteins containing specific nuclear localization signals (NLSs). We show here that either an excess or a lack of Importin alpha blocks nuclear envelope ( ...

Nature Publishing Group2004

Controlled Immobilization of Membrane Proteins to Surfaces for Fourier Transform Infrared Investigations

Horst Vogel, Per Rigler

We show that it is possible to immobilize membrane proteins uniformly and reversibly as self-assembled (sub)monolayers on nitrilotriacetic acid-covered sensor surfaces via hexahistidine sequences present either in the protein or in lipid membranes. Fourier ...

2004

Amorphous membrane blending

Christof Teuscher

The von Neumann architecture was first expressed in 1945 and has largely dominated in many variants and refinements computer science for more than half a century. Alternative architectures always occupied a marginal place only, despite a growing need for n ...

EPFL2004