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Bypassing bacterial infection in phage display by sequencing DNA released from phage particles
Related publications (78)
Multi-well plate lid for single-step pooling of 96 samples for high-throughput barcode-based sequencing
Bart Deplancke, Daniel Migliozzi, Gilles Weder, Riccardo Dainese, Daniel Alpern, Hüseyin Baris Atakan, Mustafa Demir, Dariia Gudkova
High-throughput transcriptomics is of increasing fundamental biological and clinical interest. The generation of molecular data from large collections of samples, such as biobanks and drug libraries, is boosting the development of new biomarkers and treatm ...
Dordrecht2024Solid-State Nanopores for Biomolecular Analysis and Detection
Advances in nanopore technology and data processing have rendered DNA sequencing highly accessible, unlocking a new realm of biotechnological opportunities. Commercially available nanopores for DNA sequencing are of biological origin and have certain disad ...
Springer Link2024Optimised set of oligonucleotides for bulk rna barcoding and sequencing
Bart Deplancke, Vincent Roland Julien Gardeux, Riccardo Dainese, Daniel Alpern
The present invention relates generally to the field of nucleic acid sequencing and provides oligonucleotide molecules and barcodes contained therein. These oligonucleotide molecules and barcodes molecules are useful in sequencing to identify and resolve e ...
2023Systematic benchmarking of single-cell ATAC-sequencing protocols
Bart Deplancke, Jörn Pezoldt, Camille Lucie Germaine Lambert
Benchmarking of scATAC-seq protocols demonstrates the effects of data quality on downstream analyses. Single-cell assay for transposase-accessible chromatin by sequencing (scATAC-seq) has emerged as a powerful tool for dissecting regulatory landscapes and ...
NATURE PORTFOLIO2023A field-capable rapid plant DNA extraction protocol using microneedle patches for botanical surveying and monitoring
Sebastian Maerkl, Nicolas Rémi Adam, Jonathan Selz
PremiseA novel protocol for rapid plant DNA extraction using microneedles is proposed, which supports botanic surveys, taxonomy, and systematics. This protocol can be conducted in the field with limited laboratory skills and equipment. The protocol is vali ...
WILEY2023Inferring Transmission Fitness Advantage of SARS-CoV-2 Variants of Concern in Wastewater Using Digital PCR
Tamar Kohn, Xavier Fernandez Cassi, Timothy R. Julian
Throughout the global COVID-19 pandemic, SARS-CoV-2 genetic variants of concern (VOCs) have repeatedly and independently arisen. VOCs are characterized by increased transmissibility, increased virulence, or reduced neutralization by antibodies obtained fro ...
2022Bubble-enhanced ultrasonic microfluidic chip for rapid DNA fragmentation
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DNA fragmentation is an essential process in developing genetic sequencing strategies, genetic research, as well as for the diagnosis of diseases with a genetic signature like cancer. Efficient on-chip DNA fragmentation protocols would be beneficial to pro ...
ROYAL SOC CHEMISTRY2022Polydimethylsiloxane microstructure-induced acoustic streaming for enhanced ultrasonic DNA fragmentation on a microfluidic chip
Martinus Gijs, Thomas Lehnert, Lin Sun
Next-generation sequencing (NGS) is an essential technology for DNA identification in genomic research. DNA fragmentation is a critical step for NGS and doing this on-chip is of great interest for future integrated genomic solutions. Here we demonstrate fa ...
ROYAL SOC CHEMISTRY2022Technological and computational advances driving high-throughput oncology
Christoph Merten, Alexis Louis Autour, Xiaoli Ma, Leonie Kolmar, Blandine Anne Catherine Vergier
Engineering and computational advances have opened many new avenues in cancer research, particularly when being exploited in interdisciplinary approaches. For example, the combination of microfluidics, novel sequencing technologies, and computational analy ...
ELSEVIER SCIENCE LONDON2022The enhancement of DNA fragmentation in a bench top ultrasonic water bath with needle-induced air bubbles: Simulation and experimental investigation
Martinus Gijs, Thomas Lehnert, Yang Liu, Lin Sun
Shearing DNA to a certain size is the first step in many medical and biological applications, especially in next-generation gene sequencing technology. In this article, we introduced a highly efficient ultrasonic DNA fragmentation method enhanced by needle ...
AIP Publishing2022