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The present invention describes an adhesive tape stripping transfer method for quick and non-invasive diagnosis of skin diseases by MALDI-TOF mass spectrometry analysis. Cells from the epidermis layer of a suspicious skin area of a patient are collected using adhesive tapes via at least one application of tape stripping procedure. The adhesive tapes carrying collected skin cells are analyzed directly with MALDI-TOF MS without any sample pretreatment like cell lysis or preparatory cellular component extraction. Detection and diagnosis of human and mouse melanoma are illustrated as examples in the present invention.
Christof Holliger, Alexander Mathis, Emmanuelle Rohrbach, Laetitia Janine Andrée Cardona
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Biranche Tandon, Nicolas Tissot