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Correlation of fluorescence microscopy, electron microscopy, and NanoSIMS stable isotope imaging on a single tissue section

Related publications (80)

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Virtual High-Framerate Microscopy of the Beating Heart via Sorting of Still Images

Michael Stefan Daniel Liebling, Olivia Mariani

Microscopy of the beating heart in embryos provides key insights for the study of its development. However, achieving a sufficiently high framerate is difficult with conventional cameras. Here, we present a method to reconstruct an image sequence covering ...

Idiap2019

Manipulation of redox signaling in mitochondria with trialkylphosphine chemical probes

Pablo Rivera Fuentes

Subcellular organelles maintain distinct redox environments to carry out their normal physiol. functions. Disruption of redox homeostasis, either in the oxidative or reductive direction, is linked to disease. We recently showed that trialkylphosphine-based ...

American Chemical Society2019

Block Face Scanning Electron Microscopy of Fluorescently Labeled Axons Without Using Near Infra-Red Branding

Graham Knott, Catherine Maclachlan

In this article, we describe the method that allows fluorescently tagged structures such as axons to be targeted for electron microscopy (EM) analysis without the need to convert their labels into electron dense stains, introduce any fiducial marks, or ima ...

2018

Compact lensless digital holography

Manon Rostykus

Microscopy is of high interest for biology since it allows imaging features that are too small to be seen with naked eyes. However, cells are mostly transparent to visible and infrared light which makes it difficult to see with a traditional microscope. To ...

EPFL2018

Under homoeostatic conditions, the relationship between the coralPocillopora damicornisandVibrio coralliilyticusiscommensal. An increase in temperature, or in the abundance ofV. coralliilyticus, can turn this association pathogenic,causing tissue lysis, ex ...

2018

Spinning-disc confocal microscopy in the second near-infrared window (NIR-II)

Ardemis Anoush Boghossian, Benjamin Paul Johanès Gabriel Lambert, Alessandra Antonucci, Vitalijs Zubkovs

Fluorescence microscopy in the second near-infrared optical window (NIR-II, 1000–1350 nm) has become a technique of choice for non-invasive in vivo imaging. The deep penetration of NIR light in living tissue, as well as negligible tissue autofluorescence w ...

2018

Combined Multi-Plane Tomographic Phase Retrieval and Stochastic Optical Fluctuation Imaging for 4D Cell Microscopy

Theo Lasser, Hilal Lashuel, Anne-Laure Mahul Mellier, Marcel Leutenegger, Emrah Bostan, Arno Pino Bouwens, Tomas Lukes, Adrien Charles-François Raymond Descloux

Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going beyond the diffraction barrier comes at a price since most far-field super-resolution imaging techniques trade temporal for spa ...

2018

Understanding cell functions is the major goal of molecular biology, which intends to elucidate the interactions between biomolecules at a subcellular level. One of the widely used techniques in molecular biology is fluorescence microscopy, which offers hi ...

EPFL2017

High-throughput three-dimensional cryogenic imaging of thick biological specimens is valuable for identifying biologically-or pathologically-relevant features of interest, especially for subsequent correlative studies. Unfortunately, high-resolution imagin ...

Springer Nature2017

Microscopy imaging, including fluorescence microscopy and electron microscopy, has a prominent role in life science and medical research. During the past two decades, biological imaging has undergone a revolution by way of the development of new microscopy ...

IEEE2016