Publication
Investigating receptor-mediated antibody transcytosis using blood-brain barrier organoid arrays
Related publications (32)
Single-Cell Droplet Microfluidic Screening for Antibodies Specifically Binding to Target Cells
Monoclonal antibodies are a main player in modern drug discovery. Many antibody screening formats exist, each with specific advantages and limitations. Nonetheless, it remains challenging to screen antibodies for the binding of cell-surface receptors (the ...
2018Introducing tolerance to foreign and partly foreign proteins by erythrocyte binding and artificial glycosylations
Over the last few years, protein drugs have steadily gained importance in the clinic. Compared to small molecule drugs, protein drugs are taken up by antigen presenting cells, which degrade the proteins into peptides that are then presented on major histoc ...
EPFL2018Real-time binding kinetics of ligand-receptor interactions
In principle, a major goal in drug development is to design a high-affinity compound with an appropriate pharmacological response, which requires understanding of physical processes underlying the drug-target interaction, affinity and specificity. This dru ...
EPFL2017A droplet-based heterogeneous immunoassay for screening single cells secreting antigen-specific antibodies
Samin Akbari, Tohid Pirbodaghi
High throughput heterogeneous immunoassays that screen antigen-specific antibody secreting cells are essential to accelerate monoclonal antibody discovery for therapeutic applications. Here, we introduce a heterogeneous single cell immunoassay based on alg ...
Royal Soc Chemistry2014A novel encapsulation device for peripheral implantation of antibody-secreting cells
Alzheimer’s disease (AD) is the most prevalent form of dementia in the elderly. As populations are aging in most parts of the world, the number of people with Alzheimer’s disease is expected to dramatically increase. This devastating neurodegenerative diso ...
EPFL2013Functional single-cell hybridoma screening using droplet-based microfluidics
Monoclonal antibodies can specifically bind or even inhibit drug targets and have hence become the fastest growing class of human therapeutics. Although they can be screened for binding affinities at very high throughput using systems such as phage display ...
2012Large-Scale Transfection of Mammalian Cells
Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Carine Meerschman
The large-scale transfection of mammalian cells allows moderate (milligram to gram) amounts of recombinant proteins (r-proteins) to be obtained for fundamental or clinical research. In this article, we describe a one-liter transfection using polyethyleneim ...
Humana Press2011Chronic delivery of antibody fragments using immunoisolated cell implants as a passive vaccination tool
Rolf Gruetter, Maria del Carmen Sandi Perez, Patrick Aebischer, Maurizio Molinari, Maria Isabel Cordero Campana, Jean-Charles Bensadoun, Nicolas Bouche, Veronica Setola, Osiris Marroquin Belaunzaran
BACKGROUND: Monoclonal antibodies and antibody fragments are powerful biotherapeutics for various debilitating diseases. However, high production costs, functional limitations such as inadequate pharmacokinetics and tissue accessibility are the current pri ...
Public Library of Science2011Transient Gene Expression in HEK-293E Cells for Recombinant Protein Production
The growing demand of biopharmaceutical products is boosting the market for therapeutic recombinant proteins (r-proteins). More than half of the 140 r-proteins that have gained approval for human therapeutic use are manufactured in mammalian cells that mak ...
EPFL2010Magnetic beads retention device for sandwich immunoassay: comparison of off-chip and on-chip antibody incubation
Martinus Gijs, Caroline Vandevyver, Frédéric Lacharme
We use magnetic microbeads, which are magnetically self-assembled in chains in a microfluidic chip, as reaction substrates to implement two different sandwich immunoassay protocols for the detection of mouse monoclonal target antibodies. The magnetic chain ...
Springer-Verlag2009