Tandem mass spectrometryTandem mass spectrometry, also known as MS/MS or MS2, is a technique in instrumental analysis where two or more mass analyzers are coupled together using an additional reaction step to increase their abilities to analyse chemical samples. A common use of tandem MS is the analysis of biomolecules, such as proteins and peptides. The molecules of a given sample are ionized and the first spectrometer (designated MS1) separates these ions by their mass-to-charge ratio (often given as m/z or m/Q).
Ion sourceAn ion source is a device that creates atomic and molecular ions. Ion sources are used to form ions for mass spectrometers, optical emission spectrometers, particle accelerators, ion implanters and ion engines. Electron ionization Electron ionization is widely used in mass spectrometry, particularly for organic molecules. The gas phase reaction producing electron ionization is M{} + e^- -> M^{+\bullet}{} + 2e^- where M is the atom or molecule being ionized, e^- is the electron, and M^{+\bullet} is the resulting ion.
Electron ionizationElectron ionization (EI, formerly known as electron impact ionization and electron bombardment ionization) is an ionization method in which energetic electrons interact with solid or gas phase atoms or molecules to produce ions. EI was one of the first ionization techniques developed for mass spectrometry. However, this method is still a popular ionization technique. This technique is considered a hard (high fragmentation) ionization method, since it uses highly energetic electrons to produce ions.
Mass spectral interpretationMass spectral interpretation is the method employed to identify the chemical formula, characteristic fragment patterns and possible fragment ions from the mass spectra. Mass spectra is a plot of relative abundance against mass-to-charge ratio. It is commonly used for the identification of organic compounds from electron ionization mass spectrometry. Organic chemists obtain mass spectra of chemical compounds as part of structure elucidation and the analysis is part of many organic chemistry curricula.
Electron massIn particle physics, the electron mass (symbol: me) is the mass of a stationary electron, also known as the invariant mass of the electron. It is one of the fundamental constants of physics. It has a value of about 9.109e−31kilograms or about 5.486e−4daltons, which has an energy-equivalent of about 8.187e−14joules or about The term "rest mass" is sometimes used because in special relativity the mass of an object can be said to increase in a frame of reference that is moving relative to that object (or if the object is moving in a given frame of reference).
Fourier-transform ion cyclotron resonanceFourier-transform ion cyclotron resonance mass spectrometry is a type of mass analyzer (or mass spectrometer) for determining the mass-to-charge ratio (m/z) of ions based on the cyclotron frequency of the ions in a fixed magnetic field. The ions are trapped in a Penning trap (a magnetic field with electric trapping plates), where they are excited (at their resonant cyclotron frequencies) to a larger cyclotron radius by an oscillating electric field orthogonal to the magnetic field.
Square rootIn mathematics, a square root of a number x is a number y such that ; in other words, a number y whose square (the result of multiplying the number by itself, or ) is x. For example, 4 and −4 are square roots of 16 because . Every nonnegative real number x has a unique nonnegative square root, called the principal square root, which is denoted by where the symbol "" is called the radical sign or radix. For example, to express the fact that the principal square root of 9 is 3, we write .
Magnetic pressureIn physics, magnetic pressure is an energy density associated with a magnetic field. In SI units, the energy density of a magnetic field with strength can be expressed as where is the vacuum permeability. Any magnetic field has an associated magnetic pressure contained by the boundary conditions on the field. It is identical to any other physical pressure except that it is carried by the magnetic field rather than (in the case of a gas) by the kinetic energy of gas molecules.
Square root of 2The square root of 2 (approximately 1.4142) is a positive real number that, when multiplied by itself, equals the number 2. It may be written in mathematics as or . It is an algebraic number, and therefore not a transcendental number. Technically, it should be called the principal square root of 2, to distinguish it from the negative number with the same property. Geometrically, the square root of 2 is the length of a diagonal across a square with sides of one unit of length; this follows from the Pythagorean theorem.
Fluid dynamicsIn physics, physical chemistry and engineering, fluid dynamics is a subdiscipline of fluid mechanics that describes the flow of fluids—liquids and gases. It has several subdisciplines, including aerodynamics (the study of air and other gases in motion) and hydrodynamics (the study of liquids in motion). Fluid dynamics has a wide range of applications, including calculating forces and moments on aircraft, determining the mass flow rate of petroleum through pipelines, predicting weather patterns, understanding nebulae in interstellar space and modelling fission weapon detonation.
