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Visualizing intracellular nanostructures of living cells by nanoendoscopy-AFM

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Low-dimensional perovskite nanoplatelet synthesis using in situ photophysical monitoring to establish controlled growth

Perovskite nanoparticles have attracted the attention of research groups around the world for their impressive photophysical properties, facile synthesis and versatile surface chemistry. Here, we report a synthetic route that takes advantage of a suite of ...

2019

Three-dimensional imaging of integrated circuits with macro- to nanoscale zoom

Gabriel Aeppli, Manuel Guizar Sicairos, Christian David, Michal Odstrcil

The imaging of integrated circuits across different length scales is required for failure analysis, design validation and quality control. At present, such inspection is accomplished using a hierarchy of different probes, from optical microscopy on the mil ...

NATURE PUBLISHING GROUP2019

Photorelease of caged compounds is among the most powerful experimental approaches for studying cellular functions on fast timescales. However, its full potential has yet to be exploited, as the number of caged small molecules available for cell biological ...

2019

Lignin is a phenylpropanoid polymer produced in the secondary cell walls of vascular plants. Although most eudicot and gymnosperm species generate lignins solely via polymerization of p-hydroxycinnamyl alcohols (monolignols), grasses additionally use a fla ...

2019

Fluorescent Nanodiamond–Gold Hybrid Particles for Multimodal Optical and Electron Microscopy Cellular Imaging

Theo Lasser, Weina Liu, Miguel Sison

There is a continuous demand for imaging probes offering excellent performance in various microscopy techniques for comprehensive investigations. of cellular processes by more than, one technique. FluoreScent nanodianiond gold nanoparticles (END Au) consti ...

Amer Chemical Soc2016

By live-cell imaging of biological samples dynamic cellular processes can be resolved. Fluorescence microscopy (FM) and atomic force microscopy (AFM) are both capable of imaging live cells. By combining these techniques structural as well as functional inf ...

EPFL2016

Fluorogenic Probes for Multicolor Imaging in Living Cells

Kai Johnsson, Luc Reymond, Grazvydas Lukinavicius, Olivier Vincent Sallin, Keitaro Umezawa

Here we present a far-red, silicon-rhodamine-based fluorophore (SiR700) for live-cell multicolor imaging. SiR700 has excitation and emission maxima at 690 nm and 715 nm, respectively. SiR700-based probes for F-actin, microtubules, lysosomes and SNAP-tag ar ...

American Chemical Society2016

Cell Division by Longitudinal Scission in the Insect Endosymbiont

Bruno Lemaitre, Graham Knott, Catherine Maclachlan, Elodie Julie Virginie Ramond

Spiroplasma bacteria are highly motile bacteria with no cell wall and a helical morphology. This clade includes many vertically transmitted insect endosymbionts, including Spiroplasma poulsonii, a natural endosymbiont of Drosophila melanogaster. S. poulson ...

American Society for Microbiology2016

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Nanoscale characterization of living samples has become essential for modern biology. Atomic force microscopy (AFM) creates topological images of fragile biological structures from biomolecules to living cells in aqueous environments. However, correlating ...

Amer Chemical Soc2015

In the last two decades, the third-dimension has become a focus of attention in electron microscopy to better understand the interactions within subcellular compartments. Initially, transmission electron tomography (TEM tomography) was introduced to image ...

Elsevier2015