Development of a new reporter system for the detection of protein-protein interactions in living cells
Graph Chatbot
Chat with Graph Search
Ask any question about EPFL courses, lectures, exercises, research, news, etc. or try the example questions below.
DISCLAIMER: The Graph Chatbot is not programmed to provide explicit or categorical answers to your questions. Rather, it transforms your questions into API requests that are distributed across the various IT services officially administered by EPFL. Its purpose is solely to collect and recommend relevant references to content that you can explore to help you answer your questions.
Alpha-synuclein (α-syn) is a natively unfolded protein that is closely linked to Parkinson’s disease (PD) by genetic, neuropathologic and biochemical evidence. Aggregated and fibrillar forms of α-syn are the main components of intracellular protein inclusi ...
Mesoporous silicates (MPS) provide a means of immobilizing proteins and enzymes in a stable environment while retaining physiological function. A systematic methodology of assessing the potential of immobilising a given protein on to MPS has been developed ...
Recently the sequencing project of the human genome has been completed. The interest of the post-sequencing-era is shifting nowadays towards the investigation of the biological function and localization of the encoded proteins. Proteins are essential parts ...
The identification of all protein targets of a small molecule drug, i.e. target deconvolution, provides the basis for understanding its beneficial or deleterious actions. Target deconvolution remains a major and often problematic task within the biotechnol ...
Genetically encoded fluorescent sensor proteins offer the possibility to probe the concentration of key metabolites in living cells. The approaches currently used to generate Such fluorescent sensor proteins lack. generality, as they require a protein that ...
Protein microarrays are an attractive approach for the high-throughput analysis of protein function, but their impact on proteomics has been limited by the technical difficulties associated with their generation. Here we demonstrate that fusion proteins of ...
With the human genome and many other eukaryotic and procaryotic genomes sequenced, the interest in life sciences is shifting towards the examination of the function, modification and regulation of the encoded proteins. Proteins are involved in virtually al ...
A new method for the covalent and specific labeling of fusion proteins of carrier proteins (CPs) with small organic molecules has been developed in this work. This technology combines the convenience of expressing genetically tagged reporter proteins with ...
A combinatorial method for the generation of new split-protein sensors that identifies sites at which a functional domain can be split to give two moieties that can be reconstituted to give a reporter or selectable activity is described. The method is demo ...
(Ecole Polytechnique Federale De Lausanne, Switz.).2005
The invention concerns a combinatorial method for the generation of new split-protein sensors, and its application towards the (beta/alpha)8-barrel enzyme N-(5'-phosphoribosyl)-anthranilate isomerase Trp1p from Saccharomyces cerevisiae is demonstrated. The ...
