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Application of CMOS image sensors in optical sectioning and polarization microscopy

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Systematic Tuning of Rhodamine Spirocyclization for Super-resolution Microscopy

Kai Johnsson, Lu Wang

Rhodamines are the most important class of fluorophores for applications in live-cell fluorescence microscopy. This is mainly because rhodamines exist in a dynamic equilibrium between a fluorescent zwitterion and a nonfluorescent but cell-permeable spirocy ...

AMER CHEMICAL SOC2021

Additive manufacturing of a precious bulk metallic glass

Roland Logé, Jamasp Jhabvala, Antonia Neels, Annapaola Parrilli, Güven Kurtuldu, Navid Sohrabi

For the first time, a high-density amorphous and crack-free bulk metallic glass (BMG) based on a precious metal (PdCuNiP) was produced via additive manufacturing (AM). Laser powder-bed fusion (LPBF) was used for the fabrication of the samples, and led to a ...

2021

Weakly Supervised Deep Learning Methods for Biomicroscopy

Adrian Shajkofci

Optical microscopy, an invaluable tool in biology and medicine to observe and quantify cellular function, organ development, or disease mechanisms, requires constant trade-offs between spatial, temporal, and spectral resolution, invasiveness, acquisition t ...

EPFL2021

Correlative 3D microscopy of single cells using super-resolution and scanning ion-conductance microscopy

Aleksandra Radenovic, Georg Fantner, Vytautas Navikas, Adrien Charles-François Raymond Descloux, Samuel Mendes Leitão, Kristin Stefanie Grussmayer

High-resolution live-cell imaging is necessary to study complex biological phenomena. Modern fluorescence microscopy methods are increasingly combined with complementary, label-free techniques to put the fluorescence information into the cellular context. ...

2021

Polarization-sensitive optics diffraction tomography

Demetri Psaltis, Joowon Lim, Elizabeth Elena Antoine, Amirhossein Saba Shirvan, Ahmed Ayoub

Polarization of light has been widely used as a contrast mechanism in two-dimensional (2D) microscopy and also in some three-dimensional (3D) imaging modalities. In this paper, we report the 3D tomographic reconstruction of the refractive index (RI) tensor ...

OPTICAL SOC AMER2021

Parameter-free rendering of single-molecule localization microscopy data for parameter-free resolution estimation

Aleksandra Radenovic, Adrien Charles-François Raymond Descloux, Kristin Stefanie Grussmayer

Localization microscopy is a super-resolution imaging technique that relies on the spatial and temporal separation of blinking fluorescent emitters. These blinking events can be individually localized with a precision significantly smaller than the classic ...

2021

Single-molecule localization microscopy

Suliana Manley, Juliette Griffie

Single-molecule localization microscopy (SMLM) describes a family of powerful imaging techniques that dramatically improve spatial resolution over standard, diffraction-limited microscopy techniques and can image biological structures at the molecular scal ...

SPRINGERNATURE2021

SPAD array technology enables fluctuation-contrast super-resolution in a confocal microscope

Edoardo Charbon, Claudio Bruschini, Ivan Michel Antolovic

A fluorescence correlation contrast is a straightforward approach to super-resolution imaging. Combining a SPAD array with a novel detection scheme (ISM), we obtain images with up to x4 times resolution enhancement. ...

OSA-SPIE2021

Optical diffraction tomography from single-molecule localization microscopy

Michaël Unser, Thanh-An Michel Pham, Emmanuel Emilien Louis Soubies, Ferréol Arnaud Marie Soulez

Single-molecule localization microscopy (SMLM) is a powerful method for the imaging of cellular structures. This modality delivers nanoscale resolution by sequentially activating a subset of fluorescent molecules and by extracting their super-resolved posi ...

2021

Organisation and dynamics of mitochondria and their RNA.

Timo Henry René Rey

The organisation of molecules into dynamic cells, and collaboration of many of those cells over a billion years led to the evolution of human life. During the last century, biologists then began to unravel the marvels of cellular organisation with ever in ...

EPFL2021