Fabrication of a microfluidic cell analyzer in a microchannel using impedance spectroscopy
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Perhaps no clinical field stands to gain more from gene therapy than that of lympho-hematological disorders. First, blood cells are the site of manifestation of a large number of well-characterized monogenic hereditary defects, the correction of which woul ...
Red blood cell (RBC) parameters such as morphology, volume, refractive index, and hemoglobin content are of great importance for diagnostic purposes. Existing approaches require complicated calibration procedures and robust cell perturbation. As a result, ...
This work presents label-free separation of cells in an on-chip flow cytometer based on impedance spectroscopy. The amplitude, opacity, and phase information can be used for discrimination depending on the cell types. Red blood cells, ghosts, and fixed cel ...
Background: The microfabricated impedance spectroscopy flow cytometer used in this study permits rapid dielectric characterization of a cell population with a simple microfluidic channel. Impedance measurements over a wide frequency range provide informati ...
Using a novel noncontact technique based on optical interferometry, we quantify the nanoscale thermal fluctuations of red blood cells (RBCs) and giant unilamellar vesicles (GUVs). The measurements reveal a nonvanishing tension coefficient for RBCs, which i ...
In this thesis, a new microfabricated flow cytometer for single cell discrimination based on dielectric spectroscopy is presented. Integrated tools for rapid in-flow cell handling are also demonstrated. Signal amplification and analysis as well as the chip ...
The cytokine hormones prolactin and erythropoietin mediate tissue-specific developmental outcomes by activating their cognate receptors, prolactin receptor (PrlR) and erythropoietin receptor (EpoR), respectively. The EpoR is essential for red blood cell fo ...
On-chip cell handling and analysis is given more and more interest, as new cytometry devices can be realised or efficiently combined and integrated. The developed sensor presents several improvements compared to other integrated flow cytometers. It is aime ...