Polymerase chain reactionThe polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample of DNA (or a part of it) sufficiently to enable detailed study. PCR was invented in 1983 by American biochemist Kary Mullis at Cetus Corporation; Mullis and biochemist Michael Smith, who had developed other essential ways of manipulating DNA, were jointly awarded the Nobel Prize in Chemistry in 1993.
Primer (molecular biology)A primer is a short single-stranded nucleic acid used by all living organisms in the initiation of DNA synthesis. DNA polymerase (responsible for DNA replication) enzymes are only capable of adding nucleotides to the 3’-end of an existing nucleic acid, requiring a primer be bound to the template before DNA polymerase can begin a complementary strand. DNA polymerase adds nucleotides after binding to the RNA primer and synthesizes the whole strand.
EnzymeEnzymes (ˈɛnzaɪmz) are proteins that act as biological catalysts by accelerating chemical reactions. The molecules upon which enzymes may act are called substrates, and the enzyme converts the substrates into different molecules known as products. Almost all metabolic processes in the cell need enzyme catalysis in order to occur at rates fast enough to sustain life. Metabolic pathways depend upon enzymes to catalyze individual steps.
SupernovaA supernova (: supernovae or supernovas) is a powerful and luminous explosion of a star. A supernova occurs during the last evolutionary stages of a massive star or when a white dwarf is triggered into runaway nuclear fusion. The original object, called the progenitor, either collapses to a neutron star or black hole, or is completely destroyed to form a diffuse nebula. The peak optical luminosity of a supernova can be comparable to that of an entire galaxy before fading over several weeks or months.
Stellar evolutionStellar evolution is the process by which a star changes over the course of time. Depending on the mass of the star, its lifetime can range from a few million years for the most massive to trillions of years for the least massive, which is considerably longer than the current age of the universe. The table shows the lifetimes of stars as a function of their masses. All stars are formed from collapsing clouds of gas and dust, often called nebulae or molecular clouds.
Bisulfite sequencingBisulfite sequencing (also known as bisulphite sequencing) is the use of bisulfite treatment of DNA before routine sequencing to determine the pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.
Red supergiantRed supergiants (RSGs) are stars with a supergiant luminosity class (Yerkes class I) of spectral type K or M. They are the largest stars in the universe in terms of volume, although they are not the most massive or luminous. Betelgeuse and Antares A are the brightest and best known red supergiants (RSGs), indeed the only first magnitude red supergiant stars. Stars are classified as supergiants on the basis of their spectral luminosity class.
StarA star is an astronomical object comprising a luminous spheroid of plasma held together by self-gravity. The nearest star to Earth is the Sun. Many other stars are visible to the naked eye at night; their immense distances from Earth make them appear as fixed points of light. The most prominent stars have been categorised into constellations and asterisms, and many of the brightest stars have proper names. Astronomers have assembled star catalogues that identify the known stars and provide standardized stellar designations.
GenomicsGenomics is an interdisciplinary field of biology focusing on the structure, function, evolution, mapping, and editing of genomes. A genome is an organism's complete set of DNA, including all of its genes as well as its hierarchical, three-dimensional structural configuration. In contrast to genetics, which refers to the study of individual genes and their roles in inheritance, genomics aims at the collective characterization and quantification of all of an organism's genes, their interrelations and influence on the organism.
SupergiantSupergiants are among the most massive and most luminous stars. Supergiant stars occupy the top region of the Hertzsprung–Russell diagram with absolute visual magnitudes between about −3 and −8. The temperature range of supergiant stars spans from about 3,400 K to over 20,000 K. The title supergiant, as applied to a star, does not have a single concrete definition. The term giant star was first coined by Hertzsprung when it became apparent that the majority of stars fell into two distinct regions of the Hertzsprung–Russell diagram.
