Evidence for slow motion in proteins by multiple refocusing of heteronuclear nitrogen/proton multiple quantum coherences in NMR
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The aim of the present thesis is to investigate several aspects of: the proteins mechanics, interprotein interactions and to study also new techniques, theoretical and technical, to obtain and analyze the force spectroscopy experiments. The first section i ...
In systems with homonuclear scalar couplings, the envelopes of spin echoes obtained with simple refocusing pulses or trains of such pulses are normally modulated so that it is difficult to extract transverse relaxation rates. It has been shown recently tha ...
Major urinary protein (MUP) is a pheromone-carrying protein of the lipocalin family. Previous studies by isothermal titrn. calorimetry (ITC) show that the affinity of MUP for the pheromone 2-methoxy-3-isobutylpyrazine (IBMP) is mainly driven by enthalpy, w ...
We explain how and under which conditions it is possible to obtain an efficient inversion of an entire sideband family of several hundred kHz using low-power, sideband-selective adiabatic pulses, and we illustrate with some experimental results how this fr ...
The envelopes of spin-echo amplitudes obtained with trains of multiple refocusing pulses are normally modulated by homonuclear scalar couplings. Echo modulations make it difficult to determine transverse relaxation rate constants R2. Provided that the puls ...
Since many biological processes occur on the μs to ms time scale, internal dynamics on that time scale may well be related to biological functionality. The characterization of internal dynamics is thus an important issue to improve our understanding of the ...
The transverse relaxation rates R (2) = 1/T (2) of protons can be determined by spin-echo sequences with multiple refocusing pulses using moderate radio-frequency field strengths and properly chosen inter-pulse delays so as to suppress echo modulations due ...
In recent years there has been a strong effort to reduce the size and power consumption of vapour cell atomic clocks [1,2]. The progress in this direction is driven by several factors such as the use low power laser diodes (VCSEL), Coherent Population Trap ...
We propose a new scheme for heteronuclear decoupling designed for fast magic-angle spinning (MAS), dubbed phase-inverted supercycled sequence for attenuation of rotary resonance (PISSARRO). Its efficiency compares favourably with CW, TPPM, SPINAL and XiX d ...
Multiple refocusing cycles can be used to extract transverse relaxation rates, R2, while homonuclear scalar couplings do not interfere. In this work, I have demonstrated the usefulness of a hybrid sequence, which is a compromise between single and multiple ...