Are you an EPFL student looking for a semester project?
Work with us on data science and visualisation projects, and deploy your project as an app on top of Graph Search.
We present optimized reaction conditions for the conversion of 2'-O-{[(triisopropylsilyl)oxy]methyl}(tom) protected uridine and adenosine nucleosides into the corresponding protected (3-15N)-labeled uridine and cytidine and (1-15N)-labeled adenosine and guanosine nucleosides. On a DNA synthesizer, the resulting 15N-labeled 2'-O-tom-protected phosphoramidite building blocks were efficiently incorporated into five selected positions of a hairpin bi-stable 32mer RNA sequence. By 2D-HSQC and HNN-COSY expts. in H2O/D2O 9:1, the 15N-signals of all base-paired 15N-labeled nucleotides could be identified and attributed to one of the two coexisting structures of 32mer RNA sequence. [on SciFinder (R)]
Francesco Stellacci, Weina Liu, Yong Zhu
Bart Deplancke, Vincent Roland Julien Gardeux, Riccardo Dainese, Daniel Alpern