Publications related to The effect of cell line, transfection procedure and reactor conditions on the glycosylation of recombinant human anti-rhesus D IGG1

A Polyoxyethylene-Substituted Bimetallic Europium Helicate for Luminescent Staining of Living Cells

Jean-Claude Bünzli, Anne-Sophie Chauvin, Caroline Vandevyver, Bo Song, Steve Comby

The homoditopic ligand

H_2L^{C3}

has been designed to form neutral triple-stranded bimetallic helicates of overall composition [

Ln_2(L^{C3})_3

]. The grafting of the polyoxyethylene fragments ensures water solubility and also favors cell penetration whi ...

2007

A virtual valve for smooth contamination-free flow switching

Philippe Renaud, Thomas Michael Braschler, Harald Van Lintel, Nicolas Demierre, Anja Kunze, Raphaël Tornay, Joël Theytaz, Mario Schlund, Grazia Loche

We present a channel geometry that allows for clean switching between different inlets of a microchip without any contamination of the inlets or the downstream flow. We drive this virtual valve with a pneumatic pressure setup that minimizes disturbance of ...

2007

New disposable tubes for rapid and precise biomass assessment for suspension cultures of mammalian cells

Florian Maria Wurm, David Hacker, Martin Jordan, Mathieu Benjamin Stettler, Nicolas Jaccard

We present a new approach for biomass assessment in cell culture using a disposable microcentrifuge tube. The specially designed tube is fitted with an upper chamber for sample loading and a lower 5 microL capillary for cell collection during centrifugatio ...

2006

Presence of Gal-alpha1,3Gal epitope on xenogeneic lines: implications for cellular gene therapy based on the encapsulation technology

Patrick Aebischer

Exposure to human serum induces the lysis of xenogeneic cells through natural antibodies and complement activation. The carbohydrate Galactose-alpha1,3-Galactose (Gal-alpha1,3-Gal) epitope, has been shown to be the principal antigenic determinant on target ...

2003

Influence of cell-line and process conditions on the glycosylation of recombinant proteins

The final glycosylation pattern of recombinant proteins might be determined by the choice of the host cell, cell culture conditions and the purification. Each step influences glycosylation and improved glycosylation of the product may thus be obtained by c ...

EPFL2002

S-phase synchronized CHO cells show elevated transfection efficiency and expression using CaPi

Florian Maria Wurm, Martin Jordan, Frédéric Grosjean

Various methods exist to transfect mammalian cells in culture. It is generally accepted that individual methods have to be optimized for each of the cell lines or cell types used. Despitethe use of optimized protocols, significant day-to-day variationsin t ...

2002

Peptide mapping and characterisation of glycation patterns of the glima 38 antigen recognised by autoantibodies in Type I diabetic patients

Steinunn Baekkeskov Hanahan

Aims/hypothesis. Glima 38 is an N-glycated neuroendocrine membrane protein of Mr 38 000, which is recognised by autoantibodies in approximately 20% of patients with Type I (insulin-dependent) diabetes mellitus. The aim of this study was to characterise the ...

Springer Verlag2000

The SPP biotech module 1: proteins for medical applications: a multidisciplinary effort for the development of a recombinant anti-RhD antibody preparation for the prevention of hemolytic disease of the newborn

Urs von Stockar, Florian Maria Wurm, Lucia Baldi Unser, Ian William Marison, Martin Jordan, Patrick Pugeaud, Philippe Pierre Girard, Lisa Hunt

A review with 0 refs. is presented regarding studies of a multidisciplinary team to establish the prophylactic principle and the manufg. process for anti-Rh D therapy based on recombinant antibody prepns. produced from Chinese hamster ovary cells. [on SciF ...

1999

Biotechnology in Lausanne. The Rh D project

Urs von Stockar, Florian Maria Wurm, Ian William Marison, Nicolas Mermod

A review is given with 2 refs. Hemolytic disease of the newborn is an often fatal condition of some newborn babies due to the immunogenicity of their Rh D pos. erythrocytes in the Rh D neg. mother. This condition can be prevented by injecting anti-Rh D ant ...

1999

The Semliki Forest virus system: receptor expression and scale-up

Horst Vogel, Rudolf Hovius, Alain Bernard

The highly efficient Semliki Forest virus (SFV) system has been applied to express several recombinant 7TM receptors and ligand-gated ion channels. High affinity binding and functional coupling to G proteins were obtained for the receptors. The ligand-gate ...

1997

The effect of cell line, transfection procedure and reactor conditions on the glycosylation of recombinant human anti-rhesus D IGG1

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A Polyoxyethylene-Substituted Bimetallic Europium Helicate for Luminescent Staining of Living Cells

A virtual valve for smooth contamination-free flow switching

New disposable tubes for rapid and precise biomass assessment for suspension cultures of mammalian cells

Presence of Gal-alpha1,3Gal epitope on xenogeneic lines: implications for cellular gene therapy based on the encapsulation technology

Influence of cell-line and process conditions on the glycosylation of recombinant proteins

S-phase synchronized CHO cells show elevated transfection efficiency and expression using CaPi

Peptide mapping and characterisation of glycation patterns of the glima 38 antigen recognised by autoantibodies in Type I diabetic patients

The SPP biotech module 1: proteins for medical applications: a multidisciplinary effort for the development of a recombinant anti-RhD antibody preparation for the prevention of hemolytic disease of the newborn

Biotechnology in Lausanne. The Rh D project

The Semliki Forest virus system: receptor expression and scale-up

New disposable tubes for rapid and precise biomass assessment for suspension cultures of mammalian cells

Presence of Gal-alpha1,3Gal epitope on xenogeneic lines: implications for cellular gene therapy based on the encapsulation technology

Influence of cell-line and process conditions on the glycosylation of recombinant proteins

Peptide mapping and characterisation of glycation patterns of the glima 38 antigen recognised by autoantibodies in Type I diabetic patients

A Polyoxyethylene-Substituted Bimetallic Europium Helicate for Luminescent Staining of Living Cells

The Semliki Forest virus system: receptor expression and scale-up

A virtual valve for smooth contamination-free flow switching

S-phase synchronized CHO cells show elevated transfection efficiency and expression using CaPi

The SPP biotech module 1: proteins for medical applications: a multidisciplinary effort for the development of a recombinant anti-RhD antibody preparation for the prevention of hemolytic disease of the newborn

Biotechnology in Lausanne. The Rh D project