Publication
Monitoring mis-acylated tRNA suppression efficiency in mammalian cells via EGFP fluorescence recovery
Related publications (32)
Accessible and highly observable gastrointestinal organoid model systems for studying host-pathogen interactions
Traditional cell cultures have long been fundamental to biological research, offering an alternative to animal models burdened by ethical constraints and procedural intricacies, often lacking relevance to human physiology and disease. Moreover, their inabi ...
EPFL2024Diverse cell-specific patterns of alternative polyadenylation in Drosophila
Most genes in higher eukaryotes express isoforms with distinct 3' untranslated regions (3' UTRs), generated by alternative polyadenylation (APA). Since 3' UTRs are predominant locations of post-transcriptional regulation, APA can render such programs condi ...
NATURE PORTFOLIO2022Annotation of spatially resolved single-cell data with STELLAR
Accurate cell-type annotation from spatially resolved single cells is crucial to understand functional spatial biology that is the basis of tissue organization. However, current computational methods for annotating spatially resolved single-cell data are t ...
2022Chemistry and Analysis of Nanoparticle-Modified Living Cells
Conventional therapeutics are often limited by their targeting ability, resulting in harmful and potential fatal side-effects for the patients. Recently, new strategies have been developed to improve target specificity of drugs in order to generate more ef ...
EPFL2020Photoactivatable Silicon Rhodamines for Super-Resolution Microscopy
Fluorescence microscopy is the method of choice to monitor dynamic processes in living cells due to its non-invasive nature. A variety of different fluorophores and labeling systems are currently used to selectively visualise structures or biomolecules of ...
EPFL2020Cell type prioritization in single-cell data
Grégoire Courtine, Gioele La Manno, Quentin Barraud, Jordan Squair, Mark Andrew Anderson, Claudia Kathe, Matthieu Pierre Gautier, Michael Alexander Skinnider, Thomas Haynes Hutson, Marco Milano
The cell types affected by biological perturbations in complex tissues are uncovered by single-cell analysis. We present Augur, a method to prioritize the cell types most responsive to biological perturbations in single-cell data. Augur employs a machine-l ...
NATURE PUBLISHING GROUP2020A Derived Positional Mapping of Inhibitory Subtypes in the Somatosensory Cortex
Henry Markram, Daniel Keller, Olivier Burri, Romain Guiet, Julie Meystre, Rahul Valiya Veettil
Obtaining a catalog of cell types is a fundamental building block for understanding the brain. The ideal classification of cell-types is based on the profile of molecules expressed by a cell, in particular, the profile of genes expressed. One strategy is, ...
FRONTIERS MEDIA SA2019Cellular Uptake and Intracellular Trafficking of Poly(N-(2-Hydroxypropyl) Methacrylamide)
Anders Meibom, Harm-Anton Klok, Graham Knott, Stéphane Laurent Escrig, Olivier Burri, Arne Seitz, Romain Guiet, Claudia Battistella
Cellular uptake and intracellular trafficking of polymer conjugates or polymer nanoparticles is typically monitored using fluorescence-based techniques such as confocal microscopy. While these methods have provided a wealth of insight into the internalizat ...
2019Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry
Olaia Maria Naveiras Torres-Quiroga, George Coukos, Mukul Girotra, Nicola Vannini, Alexandre Harari
A fine balance of quiescence, self-renewal, and differentiation is key to preserve the hematopoietic stem cell (HSC) pool and maintain lifelong production of all mature blood cells. In recent years cellular metabolism has emerged as a crucial regulator of ...
2019