Publication
BTA, a novel reagent for DNA attachment on glass and efficient generation of solid-phase amplified DNA colonies
Related publications (37)
A field-capable rapid plant DNA extraction protocol using microneedle patches for botanical surveying and monitoring
Sebastian Maerkl, Nicolas Rémi Adam, Jonathan Selz
PremiseA novel protocol for rapid plant DNA extraction using microneedles is proposed, which supports botanic surveys, taxonomy, and systematics. This protocol can be conducted in the field with limited laboratory skills and equipment. The protocol is vali ...
WILEY2023Polydimethylsiloxane microstructure-induced acoustic streaming for enhanced ultrasonic DNA fragmentation on a microfluidic chip
Martinus Gijs, Thomas Lehnert, Lin Sun
Next-generation sequencing (NGS) is an essential technology for DNA identification in genomic research. DNA fragmentation is a critical step for NGS and doing this on-chip is of great interest for future integrated genomic solutions. Here we demonstrate fa ...
ROYAL SOC CHEMISTRY2022Bubble-enhanced ultrasonic microfluidic chip for rapid DNA fragmentation
Martinus Gijs, Thomas Lehnert, Lin Sun
DNA fragmentation is an essential process in developing genetic sequencing strategies, genetic research, as well as for the diagnosis of diseases with a genetic signature like cancer. Efficient on-chip DNA fragmentation protocols would be beneficial to pro ...
ROYAL SOC CHEMISTRY2022The enhancement of DNA fragmentation in a bench top ultrasonic water bath with needle-induced air bubbles: Simulation and experimental investigation
Martinus Gijs, Thomas Lehnert, Yang Liu, Lin Sun
Shearing DNA to a certain size is the first step in many medical and biological applications, especially in next-generation gene sequencing technology. In this article, we introduced a highly efficient ultrasonic DNA fragmentation method enhanced by needle ...
AIP Publishing2022Establishing a Ternary System for Optical Monitoring of DNA-Protein Interactions with Single-Walled Carbon Nanotubes
DNA-protein interactions lie at the crux of life's essential processes. As such, various technologies have been developed to characterize these interactions. The distinct advantages of these technologies can be leveraged to study different facets of these ...
EPFL2020Generation of a Large Peptide Phage Display Library by Self-Ligation of Whole-Plasmid PCR Product
Christian Heinis, Xudong Kong, Vanessa Carle, Cristina Diaz Perlas, Kaycie Marie Butler
The success of phage display, used for developing target-specific binders based on peptides and proteins, depends on the size and diversity of the library screened, but generating large libraries of phage-encoded polypeptides remains challenging. New pepti ...
2020Simultaneous preservation of the DNA quality, the community composition and the density of freshwater oligochaetes for the development of genetically based biological indices
Introduction. Oligochaetes are recognized as valuable bioindicators of sediment quality in streams and lakes. The development of an oligochaete index based on the identification of specimens using DNA barcodes requires a method for simultaneously preservin ...
2018Simulating the Large-Scale Erosion of Genomic Privacy Over Time
Mathias Jacques Jean-Marc Humbert, Verena Wolf, Xiaoyu Shen
The dramatically decreasing costs of DNA sequencing have triggered more than a million humans to have their genotypes sequenced. Moreover, these individuals increasingly make their genomic data publicly available, thereby creating privacy threats for thems ...
IEEE COMPUTER SOC2018Bypassing bacterial infection in phage display by sequencing DNA released from phage particles
Christian Heinis, Xudong Kong, Camille Villequey
Phage display relies on a bacterial infection step in which the phage particles are replicated to perform multiple affinity selection rounds and to enable the identification of isolated clones by DNA sequencing. While this process is efficient for wild-typ ...
2017On the statistical physics of chains and rods, with application to multi-scale sequence-dependent DNA modelling
The complex mechanisms involved in cellular processes have been increasingly understood this past century and the central role of the DNA molecule has been recognized. The base pair sequence along a DNA fragment is observed not only to encode the genomic i ...
EPFL2016