Extended depth-of-focus by digital holographic microscopy

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Image scanning microscopy with a doughnut beam: signal strength and integrated intensity

We discuss the effects of image scanning microscopy using doughnut beam illumination on the properties of signal strength and integrated intensity. Doughnut beam illumination can give better optical sectioning and background rejection than Airy disk illumi ...

Optica Publishing Group2023

Advanced Techniques in Optical Diffraction Tomography

Ahmed Bassam Sayed Ayoub Mohamed Emam

In this thesis, we study the 3 challenges described above. First, we study different reconstruction techniques and assess the fidelity of each reconstruction results by means of structured illumination and phase conjugation. By reconstructing the 3D refrac ...

EPFL2022

Method and light microscope with a plurality of arrays of photon-counting detector elements

Edoardo Charbon, Claudio Bruschini, Ivan Michel Antolovic

A method for operating a light microscope comprises emitting and guiding a plurality of illumination light beams towards a specimen (6) to form a plurality of separated illumination light spots (2A, 2B, 2C, 2D) at the specimen; and guiding detection light ...

2022

High sensitivity, incoherent differential imaging

Chiara Bonati

Microscopic visualisation of optically transparent samples has been a topic of interest for several decades. Features such as density or chemical composition can influence the optical phase of transmitted light, and phase contrast can reveal these structur ...

EPFL2022

Exploiting Marginal Stability in Slow-Fast Quasilinear Dynamical Systems

Alessia Ferraro

The accurate investigation of many geophysical phenomena via direct numerical simulations is computationally not possible nowadays due to the huge range of spatial and temporal scales to be resolved. Therefore advances in this field rely on the development ...

EPFL2022

Computational methods for live heart imaging with speed-constrained microscopes

Olivia Mariani

Imaging methods to capture the beating and developing heart inside embryonic animal models such as the zebrafish are a key component for the study of fundamental biological processes such as cardiac birth defects or tissue regeneration. However, live heart ...

EPFL2021

Estimating Nonplanar Flow from 2D Motion-blurred Widefield Microscopy Images via Deep Learning

Michael Stefan Daniel Liebling, Adrian Shajkofci

Optical flow is a method aimed at predicting the movement velocity of any pixel in the image and is used in medicine and biology to estimate flow of particles in organs or organelles. However, a precise optical flow measurement requires images taken at hig ...

IEEE2021

Investigating Tissue Mechanics in vitro Using Untethered Soft Robotic Microdevices

Mahmut Selman Sakar, Ece Özelçi, Raquel Filipa Penacho Parreira

This paper presents the design, fabrication, and operation of a soft robotic compression device that is remotely powered by laser illumination. We combined the rapid and wireless response of hybrid nanomaterials with state-of-the-art microengineering techn ...

2021

Phase calibration of a basic bright-field microscope for 3D metrology of transparent samples at the nanoscale

Martinus Gijs, Daniel Migliozzi, Bingying Zhao

Optical retrieval of the structure of transparent objects at the nano-scale requires adapted methods capable of probing their interaction with suitable light. Scattering events, for instance, depend on the content and the arrangement of the medium encounte ...

SPIE-INT SOC OPTICAL ENGINEERING2021

Structured light generation by refractive-diffractive micro optical array illuminators

Maryam Yousefi

Structured light generation having broad applications in different optical fields, is the topic of this thesis. Our structured light generation strategy is based on applying periodic microoptical elements at the refraction-diffraction limit, under a focuse ...

EPFL2021

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