Unit
Laboratory of Experimental Biophysics
LaboratoryRelated publications (140)
Development of novel experimental and computational methods for three-dimensional coherent and super-resolution microscopy
Adrien Charles-François Raymond Descloux
Optical microscopy is one widely used tool to study cell functions and the interaction of molecules at a sub-cellular level. Optical microscopy techniques can be broadly divided into two categories: partially coherent and incoherent. Coherent microscopy te ...
EPFL2020Influenza A viruses use multivalent sialic acid clusters for cell binding and receptor activation
Suliana Manley, Christian Sieben
Influenza A virus (IAV) binds its host cell using the major viral surface protein hemagglutinin (HA). HA recognizes sialic acid, a plasma membrane glycan that functions as the specific primary attachment factor (AF). Since sialic acid alone cannot fulfill ...
2020Homogeneous multifocal excitation for high-throughput super-resolution imaging
Pierre Gönczy, Suliana Manley, Kyle Michael Douglass, Khalid Ashraf Mohie Ibrahim, Davide Gambarotto, Paul Guichard, Virginie Hamel, Denis Fortun, Niccolo Banterle, Dora Mahecic
Super-resolution microscopies have become an established tool in biological research. However, imaging throughput remains a main bottleneck in acquiring large datasets required for quantitative biology. Here we describe multifocal flat illumination for fie ...
NATURE PUBLISHING GROUP2020Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics
Phase separation concentrates mitochondrial RNA granules. Here Rey et al., show that mitochondrial RNA granules (MRGs) behaviour is consistent with liquid-liquid phase separation (LLPS) and their fusion coincides with mitochondrial remodelling. Mitochondri ...
NATURE RESEARCH2020Torsin ATPases influence chromatin interaction of the Torsin regulator LAP1
The inner nuclear membrane is functionalized by diverse transmembrane proteins that associate with nuclear lamins and/or chromatin. When cells enter mitosis, membrane-chromatin contacts must be broken to allow for proper chromosome segregation; yet how thi ...
ELIFE SCIENCES PUBLICATIONS LTD202025th Anniversary of STED Microscopy and the 20th Anniversary of SIM: feature introduction
This feature issue commemorating 25 years of STED microscopy and 20 years of SIM is intended to highlight the incredible progress and growth in the field of superresolution microscopy since Stefan Hell and Jan Wichmann published the article Breaking the di ...
OPTICAL SOC AMER2020Spatial control of nucleoporin condensation by fragile X-related proteins
Nucleoporins (Nups) build highly organized nuclear pore complexes (NPCs) at the nuclear envelope (NE). Several Nups assemble into a sieve-like hydrogel within the central channel of the NPCs. In the cytoplasm, the soluble Nups exist, but how their assembly ...
WILEY2020Centriole foci persist in starfish oocytes despite Polo-like kinase 1 inactivation or loss of microtubule nucleation activity
Pierre Gönczy, Nils Kalbfuss, Marie Juliette Ségolène Pierron
Centrioles must be eliminated or inactivated from the oocyte to ensure that only the two functional centrioles contributed by the sperm are present in the zygote. Such removal can occur during oogenesis, as in Drosophila, where departure of Polo kinase fro ...
2020Non-Toxic Virucidal Macromolecules Show High Efficacy Against Influenza Virus Ex Vivo and In Vivo
Francesco Stellacci, Paulo Henrique Jacob Silva, Valeria Cagno, Özgün Kocabiyik, Yong Zhu, Chiara Medaglia
Influenza is one of the most widespread viral infections worldwide and represents a major public health problem. The risk that one of the next pandemics is caused by an influenza strain is high. It is important to develop broad-spectrum influenza antiviral ...
WILEY2020Bacteria: Driving polar growth
Profiling the phenotype of 200,000 mutants revealed a new cofactor that may help a group of rod-shaped bacteria elongate and grow. ...
2020