Concept

NeutrAvidin

Résumé

NeutrAvidin protein is a deglycosylated version of chicken avidin, with a mass of approximately 60,000 daltons. As a result of carbohydrate removal, lectin binding is reduced to undetectable levels, yet biotin binding affinity is retained because the carbohydrate is not necessary for this activity. Avidin has a high pI but NeutrAvidin has a near-neutral pI (pH 6.3), minimizing non-specific interactions with the negatively-charged cell surface or with DNA/RNA. Neutravidin still has lysine residues that remain available for derivatization or conjugation. Like avidin itself, NeutrAvidin is a tetramer with a strong affinity for biotin (Kd = 10−15 M). In biochemical applications, streptavidin, which also binds very tightly to biotin, may be used interchangeably with NeutrAvidin. Avidin immobilized onto solid supports is also used as purification media to capture biotin-labelled protein or nucleic acid molecules. For example, cell surface proteins can be specifically labelled with membrane-impermeable biotin reagent, then specifically captured using a NeutrAvidin support.

Source officielle

https://en.wikipedia.org/wiki/NeutrAvidin

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