Concept
Focus stacking
Publications associées (32)
Image scanning microscopy with a doughnut beam: signal strength and integrated intensity
We discuss the effects of image scanning microscopy using doughnut beam illumination on the properties of signal strength and integrated intensity. Doughnut beam illumination can give better optical sectioning and background rejection than Airy disk illumi ...
Optica Publishing Group2023Laser engraving device for multiple laser beams using a data link for adjusting the focusing position
The present invention concerns a laser engraving device (1) for engraving a target surface (17). The laser engraving device (1) comprises: a beam splitter (7) for dividing a processing laser beam (25) into a plurality of engraving laser beams (27); a first ...
2022Laser engraving device for multiple laser beams
The present invention concerns a laser engraving device (1) for engraving a target surface (17). The laser engraving device (1) comprises: a beam splitter (7) for dividing a processing laser beam (25) into a plurality of engraving laser beams (27); a first ...
2020Development of novel experimental and computational methods for three-dimensional coherent and super-resolution microscopy
Adrien Charles-François Raymond Descloux
Optical microscopy is one widely used tool to study cell functions and the interaction of molecules at a sub-cellular level. Optical microscopy techniques can be broadly divided into two categories: partially coherent and incoherent. Coherent microscopy te ...
EPFL2020Correction of spherical surface measurements by confocal microscopy
Toralf Scharf, Wilfried Noell, Jeremy Béguelin
Refractive microlenses are nowadays widely used in optical systems. Characterizing their surface is essential to ensure their quality and to optimize their fabrication process. This is realized by optical surface profilers thanks to their vertical resoluti ...
2020Assessing microlens quality based on 3D irradiance measurement at the focal spot area
Toralf Scharf, Wilfried Noell, Jeremy Béguelin, Michail Symeonidis
During the fabrication process of microlenses, characterization is essential for two purposes: evaluate the optical quality of the element and provide surface information feedback for process optimization. However, no technique can fulfill these two object ...
2018Structural and functional brain imaging using extended-focus optical coherence tomography and microscopy
Neuroimaging techniques aim at revealing the anatomy and functional organisation of cerebral structures. Over the past decades, functional magnetic resonance imaging (fMRI) has revolutionized our understanding of human cerebral physiology through its abili ...
EPFL2018A Point-Spread-Function-Aware Filtered Backprojection Algorithm for Focal-Plane-Scanning Optical Projection Tomography
Michael Stefan Daniel Liebling
Traditional optical projection tomography requires the use of low numerical aperture objectives in order to achieve a large depth of field and approximate parallel projection geometry. However, low NA objectives suffer from poor resolution, resulting in bl ...
2016Functional and Structural Imaging with Optical Coherence Microscopy
Fluorescence microscopy techniques are well established research tools and have proven their use in a large variety of biomedical applications. Microscopic molecular contrast is achieved by imaging fluorescent dyes that bind specifically to a molecule of i ...
EPFL2013Focused light delivery and all optical scanning from a multimode optical fiber using digital phase conjugation
Demetri Psaltis, Christophe Moser, Salma Farahi
We demonstrate a digital phase conjugation technique for generating a sharp focus point at the end of a multimode optical fiber. A sharp focus is experimentally obtained at the distal end of a 200 mu m core multimode fiber. By recording the digital hologra ...
Spie-Int Soc Optical Engineering2013