Concept

Endonucléase

Publications associées (39)

Vibrio cholerae pathogenicity island 2 encodes two distinct types of restriction systems

Melanie Blokesch, David William Adams, Alexandre Lemopoulos, Grazia Vizzarro

In response to predation by bacteriophages and invasion by other mobile genetic elements such as plasmids, bacteria have evolved specialised defence systems that are often clustered together on genomic islands. The O1 El Tor strains of Vibrio cholerae resp ...

2024

Electronic and structural dynamics in DNA single strands

Benjamin Bauer

The absorption, conversion and transport of electronic energy in molecular aggregates is at the heart of many important natural and artificial photochemical systems, including organic solar cell materials, photosynthetic light-harvesting complexes and DNA ...

EPFL2021

A ribonucleoprotein transfection strategy for CRISPR/Cas9-mediated gene editing and single cell cloning in rainbow trout cells

Kristin Schirmer

Background The advent of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 technology marked the beginning of a new era in the field of molecular biology, allowing the efficient and precise creation of targeted mutations in the ge ...

BMC2021

Mechanistic insights into AMP-activated protein kinase-dependent gene expression

Caterina Collodet

AMP-activated protein kinase (AMPK) is a fundamental enzyme that controls energy homeostasis, through orchestrating the cellular response to a reduction in energy availability. Under conditions of cellular energy stress AMPK senses the decrease in ATP leve ...

EPFL2019

The evolution of multiple active site configurations in a designed enzyme

Ganna Gryn'ova, Dusan Petrovic

Developments in computational chemistry, bioinformatics, and laboratory evolution have facilitated the de novo design and catalytic optimization of enzymes. Besides creating useful catalysts, the generation and iterative improvement of designed enzymes can ...

NATURE PUBLISHING GROUP2018

Maturing Mycobacterium smegmatis peptidoglycan requires non-canonical crosslinks to maintain shape

Georg Fantner, John McKinney, Haig Alexander Eskandarian

In most well-studied rod-shaped bacteria, peptidoglycan is primarily crosslinked by penicillin-binding proteins (PBPs). However, in mycobacteria, crosslinks formed by L,D-transpeptidases (LDTs) are highly abundant. To elucidate the role of these unusual cr ...

ELIFE SCIENCES PUBLICATIONS LTD2018

Placeholder factors in ribosome biogenesis: please, pave my way

The synthesis of cytoplasmic eukaryotic ribosomes is an extraordinarily energy-demanding cellular activity that occurs progressively from the nucleolus to the cytoplasm. In the nucleolus, precursor rRNAs associate with a myriad of trans-acting factors and ...

Shared Science Publishers OG2017

A method for analysis and design of metabolism using metabolomics data and kinetic models: Application on lipidomics using a novel kinetic model of sphingolipid metabolism

Vassily Hatzimanikatis, Paolo Angelino, Howard Riezman, Georgios Savoglidis

We present a model-based method, designated Inverse Metabolic Control Analysis (IMCA), which can be used in conjunction with classical Metabolic Control Analysis for the analysis and design of cellular metabolism. We demonstrate the capabilities of the met ...

Academic Press Inc Elsevier Science2016

Role of microRNAs in tumor-associated macrophages

Tumor-associated macrophages (TAMs) infiltrate both mouse and human tumors, typically in high numbers, and display complex interactions with cancer cells, endothelial cells (ECs) and other immune cells. TAMs comprise molecularly distinct subsets. Two such ...

EPFL2016

Suppression of microRNA activity amplifies IFN-γ-induced macrophage activation and promotes anti-tumor immunity

Michele De Palma, Mario Leonardo Squadrito, Damya Laoui, Danielle Thompson, Sarah Kate Hansen

Tumour-associated macrophages (TAMs) largely express an alternatively activated (or M2) phenotype, which entails immunosuppressive and tumour-promoting capabilities. Reprogramming TAMs towards a classically activated (M1) phenotype may thwart tumour-associ ...

Nature Publishing Group2016

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