Concept

Électrophorèse sur gel

Publications associées (234)

Development of Electrokinetically Based Two-Dimensional Separation Methodologies for Proteomic Studies

Proteomic studies require analytical methods capable of separating, identifying and quantifying thousands of proteins. Consequently, developing separation methodologies with high resolving power necessary to separate complex protein samples prior to protei ...

EPFL2012

Segmented Field OFFGEL® Electrophoresis

Hubert Girault, Julien Maillard, Fernando Cortes Salazar, Dmitry Momotenko, Elena Tobolkina

A multielectrode setup for protein OFFGEL electrophoresis that significantly improves protein separation efficiency has been developed. Here, the electric field is applied by segments between seven electrodes connected in series to six independent power su ...

Wiley-Blackwell2012

Encapsulation of Huh-7 cells within alginate-poly(ethylene glycol) hybrid microspheres

Jutta Christine Wandrey, Redouan Mahou

Novel calcium alginate poly(ethylene glycol) hybrid microspheres (Ca-alg-PEG) were developed and evaluated as potentially suitable materials for cell microencapsulation. Grafting 5-13% of the backbone units of sodium alginate (Na-alg) with alpha-amine-omeg ...

Springer US2012

α-Synuclein in central nervous system and from erythrocytes, mammalian cells, and Escherichia coli exists predominantly as disordered monomer

Hilal Lashuel, Patrick Aebischer, Bernard Schneider, Niels Erik Lion, Elpida Tsika, Darren Moore, Michel Prudent, Bruno Claude Daniel Fauvet, Mohamed Bilal Fares, Philippe Guillaume Coune, Martial Mbefo Kamdem, Carole Desobry

Since the discovery and isolation of α-synuclein (α-syn) from human brains, it has been widely accepted that it exists as an intrinsically disordered monomeric protein. Two recent studies suggested that α-syn produced in Escherichia coli or isolated from m ...

2012

Versatile Route to Synthesize Heterobifunctional Poly(ethylene glycol) of Variable Functionality for Subsequent Pegylation

Jutta Christine Wandrey, Redouan Mahou

Pegylation using heterotelechelic poly(ethylene glycol) (PEG) offers many possibilities to create high-performance molecules and materials. A versatile route is proposed to synthesize heterobifunctional PEG containing diverse combinations of azide, amine, ...

Mdpi Ag2012

Materials science: Cell environments programmed with light

Matthias Lütolf

A combination of two light-induced reactions has been used to attach peptides to a polymeric gel, and then to detach them from it. This feat opens up opportunities for studying the effects of signalling molecules on cell behaviour in vitro. ...

2012

Musical Audio Source Separation Based on User-Selected F0 Track

Jean-Philippe Thiran

A system for user-guided audio source separation is presented in this article. Following previous works on time-frequency music representations, the proposed User Interface allows the user to select the desired audio source, by means of the assumed fundame ...

2012

Handling macromolecule signals in the quantification of the neurochemical profile

Rolf Gruetter, Cristina Ramona Cudalbu

In vivo localized proton magnetic resonance spectroscopy (1H MRS) became a powerful and unique technique to non-invasively investigate brain metabolism of rodents and humans. The main goal of 1H MRS is the reliable quantification of concentrations of metab ...

Ios Press2012

Phenyl-bridged polysilsesquioxane positive and negative resist for electron beam lithography

Jürgen Brugger, Vaida Auzelyte

We present and characterize an organic–inorganic hybrid sol–gel material, phenyl-bridged polysilsesquioxane (ph-PSQ), for use as a new highresolution resist for electron beam lithography (EBL). The resist has a unique characteristic as the only positive to ...

Institute of Physics2012

High-sensitive protein analysis by FESI-CEMALDI-MS

Hubert Girault, Mohammad Reza Pourhaghighi

Capillary zone electrophoresis (CZE) and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) are two techniques highly suitable for the separation and detection of intact proteins. Herein, based on the use of a recently introduced iont ...

Wiley-Blackwell2011

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