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Who Activates the Nucleophile in Ribozyme Catalysis? An Answer from the Splicing Mechanism of Group II Introns

Ursula Röthlisberger, Giulia Palermo, Lorenzo Casalino

Group II introns are Mg2+-dependent ribozymes that are considered to be the evolutionary ancestors of the eukaryotic spliceosome, thus representing an ideal model system to understand the mechanism of conversion of premature messenger RNA (mRNA) into matur ...

2016

Insight into the evolution and origin of leprosy bacilli from the genome sequence of Mycobacterium lepromatosis

Stewart Cole, Andrej Benjak, Charlotte Avanzi, Philippe Busso, Pushpendra Singh

Mycobacterium lepromatosis is an uncultured human pathogen associated with diffuse lepromatous leprosy and a reactional state known as Lucio's phenomenon. By using deep sequencing with and without DNA enrichment, we obtained the near-complete genome sequen ...

National Academy of Sciences2015

Holocene changes in climate and land use drove shifts in the diversity of testate amoebae in a subalpine pond

Mariusz Lamentowicz

Testate amoebae that inhabit peat are sensitive indicators of water table position. In this study, we used testate amoebae in sediments from a mire in the western Alps (Lac du Thyl) to: (1) reconstruct the hydrology of the site over the last 7,000 years, ( ...

Springer2013

A universal fluid cell for the imaging of biological specimens in the atomic force microscope

Giovanni Dietler, Sandor Kasas, Giovanni Longo, Charles Roduit, Livan Bladimir Alonso Sarduy, Bhaskar Saha

Recently, atomic force microscope (AFM) manufacturers have begun producing instruments specifically designed to image biological specimens. In most instances, they are integrated with an inverted optical microscope, which permits concurrent optical and AFM ...

Wiley-Blackwell2013

An ancestral regulatory mechanism underlies Hoxd gene expression in both developing genitals and digits

Nicolas Lonfat

Coordinated expression of HoxD cluster genes is crucial for the emergence and patterning of both developing digits and external genitalia. As a shared evolutionary origin has been proposed for these secondary structures, which may have accompanied the tran ...

EPFL2013

Morphological and Molecular Characterization of Adult Midgut Compartmentalization in Drosophila

Bruno Lemaitre, Bart Deplancke, Jean Philippe Boquete, Dani Osman

Although the gut is a central organ of Eumetazoans and is essential for organismal health, our understanding of its morphological and molecular determinants remains rudimentary. Here, we provide a comprehensive atlas of Drosophila adult midgut. Specificall ...

Elsevier2013

Evolution of whole genomes through inversions : models and algorithms for duplicates, ancestors, and edit scenarios

Krister Swenson

Advances in sequencing technology are yielding DNA sequence data at an alarming rate – a rate reminiscent of Moore's law. Biologists' abilities to analyze this data, however, have not kept pace. On the other hand, the discrete and mechanical nature of the ...

EPFL2009

Characterization of APOBEC3G binding to 7SL RNA

Didier Trono, Bastien Mangeat

Human APOBEC3 proteins are editing enzymes that can interfere with the replication of exogenous retroviruses such as human immunodeficiency virus (HIV), hepadnaviruses such as hepatitis B virus (HBV), and with the retrotransposition of endogenous retroelem ...

2008

Extracellular polymeric substances affecting pore-scale hydrologic conditions for bacterial activity in unsaturated soils

Dani Or

Soil bacterial cells are often found embedded in biosynthesized extracellular polymeric substances ( EPS) forming aggregates or stationary colonies attached to solid surfaces. Soil bacterial aggregation and pooling of resources offer a successful adaptatio ...

2007

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Extracellular polymeric substances affecting pore-scale hydrologic conditions for bacterial activity in unsaturated soils

Dani Or

2007