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Concept
Minicircle
Résumé
Minicircles are small (~4kb) circular replicons. They occur naturally in some eukaryotic organelle genomes. In the mitochondria-derived kinetoplast of trypanosomes, minicircles encode guide RNAs for RNA editing. In Amphidinium, the chloroplast genome is made of minicircles that encode chloroplast proteins.
Minicircles are small (~4kb) circular plasmid derivatives that have been freed from all prokaryotic vector parts. They have been applied as transgene carriers for the genetic modification of mammalian cells, with the advantage that, since they contain no bacterial DNA sequences, they are less likely to be perceived as foreign and destroyed. (Typical transgene delivery methods involve plasmids, which contain foreign DNA.) The smaller size of minicircles also extends their cloning capacity and facilitates their delivery into cells.
Their preparation usually follows a two-step procedure:
production of a 'parental plasmid' (bacterial plasmid with eukaryotic inserts) in E. coli
induction of a site-specific recombinase at the end of this process but still in bacteria. These steps are followed by the
excision of prokaryotic vector parts via two recombinase-target sequences at both ends of the insert
recovery of the resulting minicircle (vehicle for the highly efficient modification of the recipient cell) and the miniplasmid by capillary gel electrophoresis (CGE)
The purified minicircle can be transferred into the recipient cell by transfection or lipofection and into a differentiated tissue by, for instance, jet injection.
Conventional minicircles lack an origin of replication, so they do not replicate within the target cells and the encoded genes will disappear as the cell divides (which can be either an advantage or disadvantage depending on whether the application demands persistent or transient expression). A novel addition to the field are nonviral self-replicating minicircles, which owe this property to the presence of a S/MAR-Element.
Source officielle
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