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Publications associées (51)
Quantification of global microRNA abundance by selective isotachophoresis
We here present and demonstrate a novel technique based on isotachophoresis (ITP) for the quantification of global microRNA (miRNA) abundance in total RNA. We leverage the selectivity of ITP to concentrate miRNA and exclude longer RNA molecules from the fo ...
American Chemical Society2010GENETIC INSTABILITY OF THE DEHALORESPIRATION PROCESS IN DESULFITOBACTERIUM HAFNIENSE STRAIN TCE1
Christof Holliger, Julien Maillard, Aurélie Duret
Dehalorespiration is an environmental-friendly bacterial anaerobic respiration that couples reductive dechlorination of chlorinated organic pollutants with energy conservation. Tetrachloroethene (PCE) respiration is encoded by the pce gene cluster in Desul ...
2010Innate immunity against retroviral pathogens: from an ambiguous genetic self to novel therapeutic approaches
Almost half of the human genome is derived from exogenous genetic invaders, most of them related to retroviruses. This is the consequence of longstanding interactions between retroelements and higher organisms, governed by a delicate equilibrium between vi ...
Swiss Medical Publishers Ltd / EMH Swiss Medical Publishers2009Exploring the transcriptional landscape of plant circadian rhythms using genome tiling arrays
BACKGROUND: Organisms are able to anticipate changes in the daily environment with an internal oscillator know as the circadian clock. Transcription is an important mechanism in maintaining these oscillations. Here we explore, using whole genome tiling arr ...
BioMed Central2009Experimental advances in the characterization of metazoan gene regulatory networks
Gene regulatory networks (GRNs) play a vital role in metazoan development and function, and deregulation of these networks is often implicated in disease. GRNs depict the dynamic interactions between genomic and regulatory state components. The genomic com ...
2009Lack of Insertional-Deletional Polymorphism in a Collection of Mycobacterium ulcerans Isolates from Ghanaian Buruli Ulcer Patients
Mycobacterium ulcerans causes the devastating infectious skin disease Buruli ulcer and has a monomorphic population structure. The resolution of conventional genetic fingerprinting methods is therefore not sufficient for microepidemiological studies aiming ...
American Society for Microbiology2009Isolation and evaluation of novel adeno-associated virus sequences from porcine tissues
High antigenic compatibility and low toxicity is associated with xenograft transplantation of porcine tissues in immunodeficient human recipients. We hypothesized that adeno-associated viruses (AAVs) of porcine origin could be highly compatible to human ti ...
Nature Publishing Group2009Response of aged parkinsonian monkeys to in vivo gene transfer of GDNF
Patrick Aebischer, Romain Zufferey
This study assessed the potential for functional and anatomical recovery of the diseased aged primate nigrostriatal system, in response to trophic factor gene transfer. Aged rhesus monkeys received a single intracarotid infusion of MPTP, followed one week ...
2009One-step refolding and purification of disulfide-containing proteins with a C-terminal MESNA thioester
Maartje Martina Cornelia Bastings
Background Expression systems based on self-cleavable intein domains allow the generation of recombinant proteins with a C-terminal thioester. This uniquely reactive C-terminus can be used in native chemical ligation reactions to introduce synthetic groups ...
BioMed Central2008Inhibition of HIV-1 multiplication by a modified U7 snRNA inducing Tat and Rev exon skipping
The HIV-1 regulatory proteins Tat and Rev are encoded by multiply spliced mRNAs that differ by the use of alternative 3' splice sites at the beginning of the internal exon. If these internal exons are skipped, the expression of these genes, and hence HIV-1 ...
Wiley-Blackwell2007