Concept
Axonème
Publications associées (42)
Microtubule-mediated GLUT4 trafficking is disrupted in insulin-resistant skeletal muscle
Martinus Gijs, Raphaël Etienne Jean Trouillon, Martin Rainer Wohlwend
Microtubules serve as tracks for long-range intracellular trafficking of glucose transporter 4 (GLUT4), but the role of this process in skeletal muscle and insulin resistance is unclear. Here, we used fixed and live-cell imaging to study microtubule-based ...
eLIFE SCIENCES PUBL LTD2023Towards understanding centriole elimination
Centrioles are microtubule-based structures crucial for forming flagella, cilia and centrosomes. Through these roles, centrioles are critical notably for proper cell motility, signalling and division. Recent years have advanced significantly our understand ...
London2023Centriole elimination during Caenorhabditis elegans oogenesis initiates with loss of the central tube protein SAS-1
Pierre Gönczy, Coralie Busso, Alexander Woglar, Keshav Jha, Tamara Mikeladze-Dvali, Marie Juliette Ségolène Pierron
In most metazoans, centrioles are lost during oogenesis, ensuring that the zygote is endowed with the correct number of two centrioles, which are paternally contributed. How centriole architecture is dismantled during oogenesis is not understood. Here, we ...
Hoboken2023Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly
Georg Fantner, Georgios Hatzopoulos, Tatiana Favez, Oliver Hantschel, Virginie Hamel, Niccolo Banterle, Santiago Harald Andany
Centrioles are evolutionarily conserved multi-protein organelles essential for forming cilia and centrosomes. Centriole biogenesis begins with self-assembly of SAS-6 proteins into 9-fold symmetrical ring polymers, which then stack into a cartwheel that sca ...
NATURE RESEARCH2021MondoA regulates gene expression in cholesterol biosynthesis-associated pathways required for zebrafish epiboly
Cédric Gobet, Frédéric Bruno Martin Gachon, Benjamin Dieter Weger, Meltem Weger
The glucose-sensing Mondo pathway regulates expression of metabolic genes in mammals. Here, we characterized its function in the zebrafish and revealed an unexpected role of this pathway in vertebrate embryonic development. We showed that knockdown of mond ...
2020A helical inner scaffold provides a structural basis for centriole cohesion
Henning Paul-Julius Stahlberg, Davide Gambarotto, Paul Guichard, Virginie Hamel
The ninefold radial arrangement of microtubule triplets (MTTs) is the hallmark of the centriole, a conserved organelle crucial for the formation of centrosomes and cilia. Although strong cohesion between MTTs is critical to resist forces applied by ciliary ...
2020Architecture of the centriole cartwheel‐containing region revealed by cryo‐electron tomography
Henning Paul-Julius Stahlberg, Paul Guichard, Virginie Hamel
Centrioles are evolutionarily conserved barrels of microtubule triplets that form the core of the centrosome and the base of the cilium. While the crucial role of the proximal region in centriole biogenesis has been well documented, its native architecture ...
2020Neighborhood watch: tools for defining locale-dependent subproteomes and their contextual signaling activities
Transient associations between numerous organelles-e.g., the endoplasmic reticulum and the mitochondria-forge highly-coordinated, particular environments essential for cross-compartment information flow. Our perspective summarizes chemical-biology tools th ...
ROYAL SOC CHEMISTRY2020Novel features of centriole polarity and cartwheel stacking revealed by cryo-tomography
Davide Demurtas, Georgios Hatzopoulos, Sergey Nazarov, Alexandra Bezler, Paul Guichard, Veronika Nemcíková Villímová
Centrioles are polarized microtubule-based organelles that seed the formation of cilia, and which assemble from a cartwheel containing stacked ring oligomers ofSAS-6 proteins. A cryo-tomography map of centrioles from the termite flagellateTrichonymphaspp. ...
2020Live imaging screen reveals that TYRO3 and GAK ensure accurate spindle positioning in human cells
Proper spindle positioning is crucial for spatial cell division control. Spindle positioning in human cells relies on a ternary complex comprising G alpha i1-3, LGN and NuMA, which anchors dynein at the cell cortex, thus enabling pulling forces to be exert ...
Springer2019