Concept

Clonage thérapeutique

Publications associées (118)

Fetal cells for intervertebral disc regeneration

Degeneration of the intervertebral disc (IVD) is thought to be one main factor in the development of back pain. It is not a symptom by itself but can lead to painful pathological conditions. Current treatments aim at relieving pain by conservative care, me ...

EPFL2009

Novel determinants of the hematopoietic microenvironment in development and homeostasis

Olaia Maria Naveiras Torres-Quiroga

The promise of regenerative medicine depends on succeeding at generating sufficient amounts of functional, transplantable tissue by reliably differentiating pluripotent stem cells through the paths of ontogeny, or by efficiently expanding adult stem cells ...

Harvard University2009

DNA delivery by microinjection for the generation of recombinant mammalian cell lines

Florian Maria Wurm, David Hacker, Madiha Derouazi, Sébastien Chenuet

Gene transfer methods for producing recombinant cell lines are often not very efficient. One reason is that the recombinant DNA is delivered into the cell cytoplasm and only a small fraction reaches the nucleus. This chapter describes a method for microinj ...

Humana Press2009

Genotypic features of lentivirus transgenic mice

Didier Trono, Denis Duboule, Felix Naef, Frédéric Schütz, Marc-Olivier Sauvain

Lentivector-mediated transgenesis is increasingly used, whether for basic studies as an alternative to pronuclear injection of naked DNA or to test candidate gene therapy vectors. In an effort to characterize the genetic features of this approach, we first ...

American Society for Microbiology2008

A novel method of dynamic culture surface expansion improves mesenchymal stem cell proliferation and phenotype

Hicham Majd, Boris Hinz, Lara Buscemi Estefanell, Pierre Jean Arnould Wipff

Repeated passaging in conventional cell culture reduces pluripotency and proliferation capacity of human mesenchymal stem cells (MSC). We introduce an innovative cell culture method whereby the culture surface is dynamically enlarged during cell proliferat ...

2008

Mechanisms of Disease: cancer stem cells - targeting the evil twin

Classical antineoplastic treatments such as chemotherapy or radiation can efficiently eradicate the majority of proliferating and genetically unstable malignant cells within neoplastic lesions. There is increasing evidence, however, that these regimens fre ...

2008

Accelerating the development time of recombinant mammalian cell lines producing high titers of protein therapeutics

Sébastien Chenuet

The forthcoming arrival on the market of numerous protein therapeutics that require high clinical doses will foster the need for high-producing mammalian cell lines. Furthermore, pressures to reduce the development time for new therapeutics has meant more ...

EPFL2008

In Drosophila melanogaster the COM Locus Directs the Somatic Silencing of Two Retrotransposons through both Piwi-Dependent and -Independent Pathways

Background. In the Drosophila germ line, repeat-associated small interfering RNAs (rasiRNAs) ensure genomic stability by silencing endogenous transposable elements. This RNA silencing involves small RNAs of 26-30 nucleotides that are mainly produced from t ...

Public Library of Science2008

Integrins and the activation of latent transforming growth factor beta1 - an intimate relationship

Boris Hinz, Pierre Jean Arnould Wipff

Integrins are crucial for the ability of cells to sense mechanical perturbations and to transmit intracellular stress to their environment. We here review the more recently discovered role of integrins in activating the pleiotrophic cytokine transforming g ...

Elsevier2008

Direct Reprogramming of Terminally Differentiated Mature B Lymphocytes to Pluripotency

Patrick Schorderet

The therapeutic potential of embryonic stem cells (ESc) is a key scientific interest, but the ethical implications of stem cell research have been controversial and are often a major detriment to the advancement of our knowledge. Recently, several landmark ...

2008

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