Concept

Hybridation in situ en fluorescence

Publications associées (222)

Label-Free Electrochemical Detection of MicroRNA-122 in Real Samples by Graphene Modified Disposable Electrodes

In the present work, an electrochemical nucleic acid biosensor has been designed for the purpose of detection of miR-122 in real samples, i.e cell lysates. The fabrication of the biosensor has been done first by immobilization of complemantary anti-miR-122 ...

Electrochemical Soc Inc2016

User-Friendly Image-Based Segmentation and Analysis of Chromosomes

Michaël Unser, Florian Maria Wurm, Lucia Baldi Unser, Ricard Delgado Gonzalo, Virginie Sophie Uhlmann

We designed two efficient and user-friendly tools for the segmentation and analysis of images containing chromosomes or, more generally, rod-shaped elements that are spread on microscopic slides. The segmentation tool allows to automatically extract the pr ...

IEEE2016

Push-pull dioxaborine as fluorescent molecular rotor: far-red fluorogenic probe for ligand-receptor interactions

Fluorescent solvatochromic dyes and molecular rotors have attracted considerable attention as fluorogenic probes because of background-free detection of biomolecules in live cells in no-wash conditions. Herein, we introduce a push-pull boron-containing (di ...

Royal Soc Chemistry2016

Continuous quantification of HER2 expression by microfluidic precision immunofluorescence estimates HER2 gene amplification in breast cancer

Martinus Gijs, Diego Gabriel Dupouy, Ata Tuna Ciftlik, Maryse Fiche, Déborah Heintze

Chromogenic immunohistochemistry (IHC) is omnipresent in cancer diagnosis, but has also been criticized for its technical limit in quantifying the level of protein expression on tissue sections, thus potentially masking clinically relevant data. Shifting f ...

Nature Publishing Group2016

FLUORESCENCE IN SITU HYBRIDIZATION (FISH) ENHANCEMENT USING MICROFLUIDIC FLOW FOR AN ACCURATE, FAST AND ECONOMICAL ASSESSMENT OF HER2 STATUS IN BREAST CANCER

Martinus Gijs, Raphaël Etienne Jean Trouillon, Maryse Fiche, Huu Tuan Nguyen, Seiya Matsuoka

The fluorescence in situ hybridization (FISH) is the gold standard in human epidermal growth factor receptor 2 (HER2) status assessment in breast cancer. The dissemination of the technique is impeded by the cost of reagents and long experimental time. For ...

RCS Publising2016

Droplet-based microfluidics in drug discovery, transcriptomics and high-throughput molecular genetics

Christoph Merten

Droplet-based microfluidics enables assays to be carried out at very high throughput (up to thousands of samples per second) and enables researchers to work with very limited material, such as primary cells, patient's biopsies or expensive reagents. An add ...

2016

Hybridization and electron-phonon coupling in ferroelectric BaTiO3 probed by resonant inelastic x-ray scattering

Marco Grioni, Simon Karl Moser, Sara Fatale

We investigated the ferroelectric perovskite material BaTiO3 by resonant inelastic x-ray scattering (RIXS) at the Ti L-3 edge. We observe with decreasing temperature a transfer of spectral weight from the elastic to the charge-transfer spectral features, i ...

Amer Physical Soc2016

Pro-oxidant effects of nano-TiO2 on Chlamydomonas reinhardtii during short-term exposure

Olivier Martin, Paul Bowen, Christian Santschi, Lionel André Maurizi, Volodymyr Koman, Nadia Rachel Von Moos, Amarnath Jayaprakash

This study sheds light on the short-term dynamics of pro-oxidant processes related to the exposure of C. reinhardtii microalgae to nano-TiO2 using (a) conventional fluorescence probes for cellular pro-oxidant process and (b) a recently developed cytochrome ...

Royal Soc Chemistry2016

Manufacturing of orifices in glass like materials, e.g. Nanocapillaries, and objects obtained according to this process

Aleksandra Radenovic, Lorenz Jan Steinbock

The ability to reshape nanopores and observe their shrinkage under an electron microscope is a powerful and novel technique14,17. It increases the sensitivity of the resistive pulse sensing and enables to detect very short and small molecules12,31. However ...

2016

Mass Barcode Signal Amplification for Multiplex Allergy Diagnosis by MALDI-MS

Hubert Girault, Natalia Gasilova, Liang Qiao, Xiaoqin Zhong

A highly sensitive method based on mass-barcoded gold nanoparticles (AuNPs) and immunomagnetic separation has been developed for multiplex allergy diagnosis by MALDI mass spectrometry in a component-resolved manner. Different analytical probes were prepare ...

American Chemical Society2016

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