Publications associées à Hybridation in situ en fluorescence

Models that include supercoiling of topological domains reproduce several known features of interphase chromosomes

Julien Dorier, Yannis Burnier, Fabrizio Benedetti, Andrzej Stasiak

Understanding the structure of interphase chromosomes is essential to elucidate regulatory mechanisms of gene expression. During recent years, high-throughput DNA sequencing expanded the power of chromosome conformation capture (3C) methods that provide in ...

Oxford University Press2014

Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging

Theo Lasser, Marcel Leutenegger, Noelia Laura Bocchio, Aurélien Godinat, Patrick Alain Sandoz, Azat Sharipov, Stefan Geissbuehler

Super-resolution optical fluctuation imaging (SOFI) provides an elegant way of overcoming the diffraction limit in all three spatial dimensions by computing higher-order cumulants of image sequences of blinking fluorophores acquired with a classical widefi ...

2014

Junctionless Silicon Nanowire Resonator

Mihai Adrian Ionescu, Sebastian Thimotee Bartsch, Maren Arp

The development of nanoelectromechanical systems (NEMS) is likely to open up a broad spectrum of applications in science and technology. In this study, we demonstrate a novel double-transduction principle for silicon nanowire resonators which exploits the ...

2014

Programming and use of Parylene C fluorescence as a quantitative on-chip reference

Martinus Gijs, Diego Gabriel Dupouy, Ata Tuna Ciftlik, Joan Teixidor

A large number of lab-on-a-chip applications use fluorescence for quantifying biological entities. In such applications, incorporation of a stable on-chip fluorescent reference source would be highly desirable in order to compensate for instrumental parame ...

Royal Soc Chemistry2014

Structural and energetic basis for hybridization limits in high-density DNA monolayers

Alessandro Barducci

High-density monolayers (HDMs) of single-strand (ss) DNA are important nanoscale platforms for the fabrication of sensors and for mechanistic studies of enzymes on surfaces. Such systems can be used, for example, to monitor gene expression, and for the con ...

Royal Society of Chemistry2013

Substrates for Improved Live-Cell Fluorescence Labeling of SNAP-tag

Kai Johnsson, Luc Reymond, Grazvydas Lukinavicius

The SNAP-tag labeling technology provides a simple, robust, and versatile approach to the imaging of fusion proteins for a wide range of experimental applications. Owing to the specific and covalent nature of the labeling reaction, SNAP-tag is well suited ...

Bentham Science Publ Ltd2013

Microtextured Substrates and Microparticles Used as in Situ Lenses for On-Chip Immunofluorescence Amplification

Martinus Gijs, Hui Yang

We propose a concept for enhancing the fluorescent detection signal of on-chip immunoassays by using three-dimensional 3 and 8 mu m size microtextured structures as substrates for the assay, which allows exploitation of a large number of fluorophores withi ...

Amer Chemical Soc2013

SPAD array camera for localization based super resolution microscopy

Edoardo Charbon, Claudio Bruschini, Francesco Regazzoni, Samuel Burri

Super resolution microscopy by localization is a stochastic based approach, where the resolution is determined by the localization accuracy [1] [2] [3]. The accuracy of localization heavily depends on the statistics of the data obtained with a camera durin ...

2013

Multiplexed surface micropatterning of proteins with a pressure-modulated microfluidic button-membrane

Sebastian Maerkl, Jose Luis Garcia Cordero

We report the flow-based in situ patterning of multiple proteins in microfluidic channels by simply tuning the actuation pressure of a microfluidic button-membrane. We show that a single button-membrane can pattern several concentric protein annuli, and we ...

Royal Soc Chemistry2013

Chattez avec Graph Search

Posez n’importe quelle question sur les cours, conférences, exercices, recherches, actualités, etc. de l’EPFL ou essayez les exemples de questions ci-dessous.

AVERTISSEMENT : Le chatbot Graph n'est pas programmé pour fournir des réponses explicites ou catégoriques à vos questions. Il transforme plutôt vos questions en demandes API qui sont distribuées aux différents services informatiques officiellement administrés par l'EPFL. Son but est uniquement de collecter et de recommander des références pertinentes à des contenus que vous pouvez explorer pour vous aider à répondre à vos questions.

Hybridation in situ en fluorescence

Models that include supercoiling of topological domains reproduce several known features of interphase chromosomes

Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging

Anti-nicastrin monoclonal antibodies elicit pleiotropic anti-tumour pharmacological effects in invasive breast cancer cells

Junctionless Silicon Nanowire Resonator

Programming and use of Parylene C fluorescence as a quantitative on-chip reference

Structural and energetic basis for hybridization limits in high-density DNA monolayers

Substrates for Improved Live-Cell Fluorescence Labeling of SNAP-tag

Microtextured Substrates and Microparticles Used as in Situ Lenses for On-Chip Immunofluorescence Amplification

SPAD array camera for localization based super resolution microscopy

Multiplexed surface micropatterning of proteins with a pressure-modulated microfluidic button-membrane

Graph Chatbot

Chattez avec Graph Search

Anti-nicastrin monoclonal antibodies elicit pleiotropic anti-tumour pharmacological effects in invasive breast cancer cells

Microtextured Substrates and Microparticles Used as in Situ Lenses for On-Chip Immunofluorescence Amplification

Models that include supercoiling of topological domains reproduce several known features of interphase chromosomes

Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging

Junctionless Silicon Nanowire Resonator

Programming and use of Parylene C fluorescence as a quantitative on-chip reference

Structural and energetic basis for hybridization limits in high-density DNA monolayers

Substrates for Improved Live-Cell Fluorescence Labeling of SNAP-tag

SPAD array camera for localization based super resolution microscopy

Multiplexed surface micropatterning of proteins with a pressure-modulated microfluidic button-membrane