Concept

Saccharomyces

Publications associées (58)

Analysis of yeast metabolism using metabolic control analysis under uncertainty

Advancement of genomic research and anal. techniques enables an in-depth understanding of individual components in the cellular metab. The crit. subject is the integration of massive exptl. details and the quantification of the contributions of individual ...

2005

Development of a new reporter system for the detection of protein-protein interactions in living cells

Petra Tafelmeyer

Split-protein sensors have become an important tool for the analysis of protein-protein interactions in living cells. In general, two interacting proteins are expressed as fusion proteins with a pair of inactive fragments of a reporter enzyme. Interaction- ...

EPFL2004

SIN and the art of splitting the fission yeast cell

Viesturs Simanis, Andrea Krapp

The septation initiation network (SIN) triggers the onset of cytokinesis in the fission yeast Schizosaccharomyces pombe by promoting contraction of the medially placed F-actin ring. SIN signaling is regulated by the polo-like kinase plo1p and by cdc2p, the ...

2004

Amperometric monitoring of redox activity in living yeast cells:comparison of menadione sodium bisulfite as electron transfer mediators

An amperometric method was applied for real-time monitoring of intracellular redox enzyme activity. Baker's yeast (Saccharomyces cerevisiae) cells were immobilized on platinum microband electrodes and mediated anodic currents were measured. The currents we ...

2004

Control of yeast fed-batch process through regulation of extracellular ethanol concentration

Urs von Stockar, Sergio Valentinotti, Christopher Cannizzaro

At high growth rates, the biomass yield of baker's yeast (Saccharomyces cerevisiae) decreases due to the prodn. of ethanol. For this reason, it is std. industrial practice to use a fed-batch process whereby the specific growth rate, m, is fixed at a level ...

Springer-Verlag2004

Recruitment of NIMA kinase shows that maturation of the S. pombe spindle-pole body occurs over consecutive cell cycles and reveals a role for NIMA in modulating SIN activity

Viesturs Simanis, Andrea Krapp

Mitotic exit in Saccharomyces cerevisiae and septation in Schizosaccharomyces pombe are regulated by a conserved signaling network called the mitotic exit and septum initiation networks (SIN), respectively. The network is active on one of the two anaphase ...

2004

Transforming a (beta/alpha)8-Barrel Enzyme into a Split-Protein Sensor through Directed Evolution

Kai Johnsson, Petra Tafelmeyer

Split-protein sensors have become an important tool for the anal. of protein-protein interactions in living cells. We present here a combinatorial method for the generation of new split-protein sensors and demonstrate its application toward the (b/a)8-barr ...

2004

Telomere Length Homeostasis Is Achieved via a Switch between Telomerase- Extendible and -Nonextendible States

Joachim Lingner

Telomerase counteracts telomere erosion that stems from incomplete chromosome end replication and nucleolytic processing. A precise understanding of telomere length homeostasis has been hampered by the lack of assays that delineate the nonuniform telomere ...

2004

Induced Protein Dimerization in Vivo through Covalent Labeling

Kai Johnsson

The authors present here a novel approach to induce protein dimerization in living cells through covalent labeling of fusion proteins with ligands which are interacting with other proteins. The covalent labeling is based on the reaction of the DNA repair p ...

2003

Generally applicable fed-batch culture concept based on the detection of metabolic state by on-line balancing

Urs von Stockar, Ian William Marison, Christoph Herwig, Bernhard Walker

In many microorganisms, flux limitations in oxidative metab. lead to the formation of overflow metabolites even under fully aerobic conditions. This can be avoided if the specific growth rate is controlled at a low enough value. This is usually accomplishe ...

2003

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