Concept
Cryofixation
Publications associées (22)
Towards near-atomic resolution imaging of microsecond nanoscale dynamics
Time-resolved electron microscopy has made significant progress in recent years, with some groups now working on instruments that offer attosecond temporal resolution. While much of the research in the field revolves around the improvement of temporal reso ...
EPFL2023Near-atomic resolution reconstructions from in situ revitrified cryo samples
Marcel Drabbels, Ulrich Lorenz, Gabriele Bongiovanni, Jonathan Mark Voss, Oliver Florian Harder
A microsecond time-resolved version of cryo-electron microscopy (cryo-EM) has recently been introduced to enable observation of the fast conformational motions of proteins. The technique involves locally melting a cryo sample with a laser beam to allow the ...
2023Microsecond melting and revitrification of cryo samples: protein structure and beam-induced motion
Marcel Drabbels, Ulrich Lorenz, Pavel Olshin, Jonathan Mark Voss, Oliver Florian Harder
A novel approach to time-resolved cryo-electron microscopy (cryo-EM) has recently been introduced that involves melting a cryo sample with a laser beam to allow protein dynamics to briefly occur in the liquid, before trapping the particles in their transie ...
2022Rapid melting and revitrification as an approach to microsecond time-resolved cryo-electron microscopy
Marcel Drabbels, Ulrich Lorenz, Pavel Olshin, Jonathan Mark Voss, Oliver Florian Harder
Proteins typically undergo dynamics on the microsecond to millisecond timescale, which is much faster than the time resolution of cryo-electron microscopy. Here, we propose a novel approach for microsecond time-resolved cryo-electron microscopy that involv ...
2021