Concept

Microscopie par excitation à deux photons

Publications associées (489)

Confocal microscopy through a multimode fiber using optical correlation

Demetri Psaltis, Christophe Moser, Damien Claude-Marie Loterie

We report on a method to obtain confocal imaging through multimode fibers using optical correlation. First, we measure the fiber's transmission matrix in a calibration step. This allows us to create focused spots at one end of the fiber by shaping the wave ...

Optical Soc Amer2015

Digital confocal microscopy through a multimode fiber

Demetri Psaltis, Christophe Moser, Ioannis Papadopoulos, Damien Claude-Marie Loterie, Salma Farahi, Alexandre Goy

Acquiring high-contrast optical images deep inside biological tissues is still a challenging problem. Confocal microscopy is an important tool for biomedical imaging since it improves image quality by rejecting background signals. However, it suffers from ...

Optical Soc Amer2015

CIDRE: an illumination-correction method for optical microscopy

Kevin Smith, Yunpeng Li

Uneven illumination affects every image acquired by a microscope. It is often overlooked, but it can introduce considerable bias to image measurements. The most reliable correction methods require special reference images, and retrospective alternatives do ...

Nature Publishing Group2015

Estimation of Divergence-Free 3D Cardiac Blood Flow in a Zebrafish Larva Using Multi-View Microscopy

Michael Stefan Daniel Liebling

Conventional fluid flow estimation methods for in vivo optical microscopy are limited to two-dimensions and are only able to estimate the components of flow parallel to the imaging plane. This limits the study of flow in more intricate biological structure ...

2015

Fluorescent Labeling of SNAP-Tagged Proteins in Cells

Kai Johnsson, Luc Reymond, Grazvydas Lukinavicius

One of the most prominent self-labeling tags is SNAP-tag. It is an in vitro evolution product of the human DNA repair protein O6 -alkylguanine-DNA alkyltransferase (hAGT) that reacts specifically with benzylguanine (BG) and benzylchloropyrimidine (CP) deri ...

Springer2015

Multiphoton microscopy in brain imaging

Graham Knott, Bohumil Maco, Ludovico Silvestri

Brain imaging is becoming an important field in the frame of the neurophotonics in correlations with other medical ones in neuroscience studying functional and morphological aspects. In this presentation an overview on multi photon imaging of the brain wil ...

Spie-Int Soc Optical Engineering2015

Intensity-Based Point-Spread-Function-Aware Registration for Multi-View Applications in Optical Microscopy

Michael Stefan Daniel Liebling

We present an algorithm to spatially register two volumetric datasets related via a rigid-body transform and degraded by an anisotropic point-spread-function (PSF). Registration is necessary, for example, when fusing data in multi-view microscopy. Automati ...

2015

Delivery of focused short pulses through a multimode fiber

Demetri Psaltis, Christophe Moser, Salma Farahi, Edgar Emilio Morales Delgado

Light propagation through multimode fibers suffers from spatial distortions that lead to a scrambled intensity profile. In previous work, the correction of such distortions using various wavefront control methods has been demonstrated in the continuous wav ...

Optical Soc Amer2015

Quantitative evaluation of software packages for single-molecule localization microscopy

Michaël Unser, Suliana Manley, Daniel Sage, Hagay Kirshner, Thomas Pengo

The quality of super-resolution images obtained by single-molecule localization microscopy (SMLM) depends largely on the software used to detect and accurately localize point sources. In this work, we focus on the computational aspects of super-resolution ...

Nature Publishing Group2015

Optical properties of small metal clusters

Chongqi Yu

This thesis is devoted to the study of the optical properties of small metal clusters and metal cluster organic compounds. For this purpose a ultra-high vacuum (UHV) experimental apparatus has been built which allows for the deposition of mass selected neu ...

EPFL2015

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