Concept

Microscopie par excitation à deux photons

Publications associées (489)

Black Lipid Membranes: Challenges in Simultaneous Quantitative Characterization by Electrophysiology and Fluorescence Microscopy

Esther Amstad, Stefan Matile, Maria Tsemperouli

Horizontal black lipid membranes (BLMs) enable optical microscopy to be combined with the electrophysiological measurements for studying ion channels, peptide pores, and ionophores. However, a careful literature review reveals that simultaneous fluorescenc ...

AMER CHEMICAL SOC2019

Manipulation of redox signaling in mitochondria with trialkylphosphine chemical probes

Pablo Rivera Fuentes

Subcellular organelles maintain distinct redox environments to carry out their normal physiol. functions. Disruption of redox homeostasis, either in the oxidative or reductive direction, is linked to disease. We recently showed that trialkylphosphine-based ...

American Chemical Society2019

Biomedical optical techniques for intracochlear cellular imaging

Marilisa Romito

Imaging the inner ear microanatomy is of great importance for the assessment of the state of the cells responsible for sound detection. Hearing disorders are mainly due to a malfunction of the cochlea, the bone containing the cells inside the inner ear. Un ...

EPFL2019

Wide-field imaging of cortical neuronal activity with red-shifted functional indicators during motor task execution

Silvestro Micera, Maria Pasquini, Francesco Saverio Pavone

Genetically encoded calcium indicators (GECIs) can be used to monitor the intracellular concentration of free calcium in populations of neurons. GECIs with long emission wavelengths are particularly attractive for deep tissue microscopy in vivo, and have t ...

IOP PUBLISHING LTD2019

Endogenous SHG and 2PEF coherence imaging of substructures in neurons in 3D

Sylvie Roke, Carlos Alberto Macias Romero, Marie Eve Pascale Didier, Claire Jacqueline Léa Teulon

Neuronal morphology, long-distance transport and signalling critically depend on the organization of microtubules in the cytoskeleton. Second harmonic generation (SHG) imaging has been recognized as a potentially powerful tool for in situ label-free neuroi ...

2019

Scattering HCl Molecules from Au(111) and Ag(111) Surfaces

Jan Daniel Geweke

In this thesis, I present an experimental molecular beam surface scattering study of dynamical processes involved when HCl molecules are scattered from Au(111) and Ag(111) surfaces. I investigated vibrational excitation, translational inelasticity and diss ...

EPFL2019

A Large Range of Motion 3D MEMS Scanner With Five Degrees of Freedom

Matthias Imboden

Here, we discuss a novel, mixed mode 3D XYZ scanner built within a single foundry process. The device has a large range of motion in X, Y, and Z (14.1 mu m in X and Y and 97.9 mu m in Z) and can also rotate about two axes (7.4 degrees), making it a 5 degre ...

IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC2019

Waveguide-Based Platform for Large-FOV Imaging of Optically Active Defects in 2D Materials

Aleksandra Radenovic, Suliana Manley, Vytautas Navikas, Martina Lihter, Evgenii Glushkov, Anton Stroganov, Mukeshchand Thakur, Anna Archetti, Juan Francisco Gonzalez Marin, Jean Camille Benjamin Comtet

Single-molecule localization microscopy (SMLM) is a powerful tool that is routinely used for nanoscale optical imaging of biological samples. Recently, this approach has been applied to study optically active defects in two-dimensional (2D) materials. Such ...

AMER CHEMICAL SOC2019

Photoregulated fluxional fluorophores for live-cell super-resolution microscopy with no apparent photobleaching

Pablo Rivera Fuentes

Photoswitchable mols. have multiple applications in the phys. and life sciences because their properties can be modulated with light. Fluxional mols., which undergo rapid degenerate rearrangements in the electronic ground state, also exhibit switching beha ...

Springer2019

Generalized temporal sampling with active illumination in optical microscopy

Michael Stefan Daniel Liebling, Christian Jaques

Generalized sampling is a flexible framework for signal acquisition, which relaxes the need for ideal pre-filters. Nevertheless, implementation remains challenging for dynamic imaging applications because it requires simultaneously measuring multiple overl ...

SPIE2019

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