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Conventional connectors utilize mechanical, magnetic, or electrostatic interactions to enable highly specific and reversible binding of the components (i.e., mates) for a wide range of applications. As the connectors are miniaturized to small scales, a num ...
Understanding the functional dynamics of ligand-gated ion-channels (LGIC's) on the molecular level is the aim of this thesis. Members of the protein family of LGIC's are involved in the fast synaptic signal transmission and thereby play a central role in i ...
We introduce here a new class of BODIPY-based Ca2+ indicators which can be derivatized with biological ligands that permit the localization of the indicators in living cells. The underivatized BODIPY-based Ca2+ indicator (BOCA-1) shows a 250-fold increase ...
A sensitive chemiluminescence (CL)-based immunoassay technique based on both dipstick and flow injection analytical formats is reported for the detection of atrazine. In the dipstick-based immunoassay technique, antibody (anti-atrazine) was first immobiliz ...
Systemic hyalinosis is an autosomal recessive disease that encompasses two allelic syndromes, infantile systemic hyalinosis (ISH) and juvenile hyaline fibromatosis (JHF), which are caused by mutations in the CMG2 gene. Here we have analyzed the cellular co ...
Interactions between Src homology 2 (SH2) domains and phosphotyrosine sites regulate tyrosine kinase signaling networks. Selective perturbation of these interactions is challenging due to the high homology among the 120 human SH2 domains. Using an improved ...
Peptides are an attractive class of molecules for the development of therapeutics because they combine unique properties such as high binding affinity, excellent target specificity, low toxicity and a relatively small mass. However, the short in vivo half- ...
The serotonin type 3 receptor (5HT3R) is a homopentameric, cation selective channel that mediates fast excitatory transmission in the nervous system. It is a member of the Cys-loop family of receptors that include the nicotinic acetylcholine receptor (nACh ...
A specific multi-phosphopeptide enrichment method is presented to complement current phosphopeptide isolation strategies that normally bias mass spectrometry analysis towards the detection of mono-phosphopeptides. An adsorption model is developed that show ...
Electrospray ionisation mass spectrometry was used to analyze the reactions of metal compds. with mixts. of selected proteins. Three representative medicinally relevant compds., cisplatin, transplatin and the organometallic ruthenium compd. RAPTA-C, were r ...