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Densely arrayed skeletal myotubes are activated individually and as a group using precise optical stimulation with high spatiotemporal resolution. Skeletal muscle myoblasts are genetically encoded to express a light-activated cation channel, Channelrhodops ...
We demonstrate that, with appropriate staining, high-resolution X-ray microscopy can image complicated tissue structures-cerebellum and liver-and resolve large or small amounts of Au nanoparticles in these tissues. Specifically, images of tumor tissue reve ...
A 10,000-km2 hypoxic ‘dead zone’ forms, during most years, in the central basin of Lake Erie. To investigate the processes driving the hypoxia, we conducted a 2-yr field campaign where the mixing in the lake interior during the stratification period was ex ...
American Society of Limnology and Oceanography2012
Metabolic homeostasis is achieved by complex molecular and cellular networks that differ significantly among individuals and are difficult to model with genetically engineered lines of mice optimized to study single gene function. Here, we systematically a ...
Regenerative medicine aims to recreate functional tissues in vitro for the regeneration of damaged organs and solve the dramatic problem of transplant rejection. Cornea integrity is primordial for our vision. Several therapies to regenerate healthy corneal ...
Imaging rapidly changing gene expression during embryogenesis is a challenge for the development of probes and imaging techniques. The vertebrate Segmentation Clock is a genetic network that controls the subdivision of the elongating embryonic body axis in ...
This technical report provides supplementary information for the optimization problems defined for Stable Estimator of Dynamical Systems (SEDS). Reading of this report is not necessary for researchers who only want to use SEDS learning algorithm. The repor ...
Distinguishing subpopulations in group behavioral experiments can reveal the impact of differences in genetic, pharmacological and life-histories on social interactions and decision-making. Here we describe Fluorescence Behavioral Imaging (FBI), a toolkit ...
Mammalian genomes contain numerous regulatory DNA sites with unknown target genes. We used mice with an extra β-globin locus control region (LCR) to investigate how a regulator searches the genome for target genes. We find that the LCR samples a restricted ...
Mammalian cells in bioreactors as production host are the focus of this review. We wish to briefly describe today's technical status and to highlight emerging trends in the manufacture of recombinant therapeutic proteins, focusing on Chinese hamster ovary ...