Concept

Fluorescence in the life sciences

Publications associées (205)

Fura-2FF-based calcium indicator for protein labeling

We describe the synthesis and fluorescence properties of a Fura-2FF-based fluorescent Ca(2+) indicator that can be covalently linked to SNAP-tag fusion proteins and retains its Ca(2+) sensing ability after coupling to protein. ...

Royal Society of Chemistry2010

Coherent ultrafast torsional motion and isomerization of a biomimetic dipolar photoswitch

Majed Chergui, Andrea Cannizzo, Olivier Christian Bräm, Stefan Haacke

Femtosecond fluorescence up-conversion, UV-Vis and IR transient absorption spectroscopy are used to study the photo-isomerization dynamics of a new type of zwitterionic photoswitch based on a N-alkylated indanylidene pyrroline Schiff base framework (ZW-NAI ...

Royal Society of Chemistry2010

Charge transfer to solvent identified using dark channel fluorescence-yield L-edge spectroscopy

Majed Chergui, Mercedes Hannelore Rittmann-Frank

Aqueous ions are central to catalysis and biological function and play an important role in radiation biology as sources of damage-inducing electrons. Detailed knowledge of solute-solvent interactions is therefore crucial. For transition-metal ions, soft X ...

2010

A Versatile Route to Red-Emitting Carbopyronine Dyes for Optical Microscopy and Nanoscopy

Marcel Leutenegger

Biological microscopy favors photostable fluorescent markers with large fluorescence quantum yields, low dark triplet state population, good biocompatibility and absorption and emission maxima in the near-infrared, where cellular autofluorescence is minimi ...

2010

Transient State Monitoring by Total Internal Reflection Fluorescence Microscopy

Theo Lasser, Thiemo Spielmann

Triplet, photo-oxidized and other photoinduced, long-lived states of fluorophores are sensitive to the local environment and thus attractive for microenvironmental imaging purposes. In this work, we introduce an approach where these states are monitored in ...

2010

Two-Photon Excited Fluorescence Enhancement for Ultrasensitive DNA Detection on Large-Area Gold Nanopatterns

Francesco Stellacci

Ultrasensitive DNA detection using enhanced two-photon excited fluorescence: DNA detection systems formed on a highly regular gold nanopattern over a large area show enhanced sensitivity from high to ultralow concentrations (similar to 10-11 M, see figure) ...

2010

Near-field mediated enhancement of two-photon induced fluorescence on plasmonic nanostructures

Noelia Laura Bocchio

We investigate two-photon-induced fluorescence for organic chromophores next to elongated gold disks which support plasmonic resonances. By polyelectrolyte coating, the dye is placed in a defined concentration at a defined distance from the surface. A fluo ...

American Chemical Society2010

Generation of stable, high-producing cho cell lines by lentiviral vector-mediated gene transfer in serum-free suspension culture

Florian Maria Wurm, David Hacker, Agata Oberbek

Lentivirus-derived vectors (LVs) were studied for the generation of stable recombinant Chinese hamster ovary (CHO) cell lines. Stable pools and clones expressing the enhanced green fluorescent protein (eGFP) were selected via fluorescence-activated cell so ...

Wiley-Blackwell2010

Super-resolved position and orientation estimation of fluorescent dipoles using 3D steerable filters

Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler

Fluorescence localization microscopy techniques such as PALM and STORM achieve super-resolution by sequentially imaging sparse subsets of fluorophores, which are localized by means of Gaussian-based localization. Applied to fixed fluorophores, with dipole ...

2010

A new single-photon avalanche diode in 90nm standard CMOS technology

Edoardo Charbon, Marek Gersbach, Hyungjune Yoon, Mohammad Azim Karami

We report on the first implementation of a single-photon avalanche diode (SPAD) in 90nm complementary metal oxide semiconductor (CMOS) technology. The detector features an octagonal multiplication region and a guard ring to prevent premature edge breakdown ...

Optical Society of America2010

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