Concept
Réaction en chaîne par polymérase
Publications associées (233)
Electrochemical sensors for the detection of SARS-CoV-2 virus
Somanath Jagannath, Neeraj Kumar
Coronavirus (COVID-19), a deadly pandemic has spread worldwide and created many global health issues. Though methods of its detection are being continuously developed for the early detection and monitoring of COVID-19, still there is need for more novel me ...
ELSEVIER SCIENCE SA2022Development and validation of a multiplex real-time qPCR assay using GMP-grade reagents for leprosy diagnosis
Stewart Cole, Charlotte Avanzi, Thyago Leal Calvo
Author summaryLeprosy is a chronic dermato-neurological disease caused by Mycobacterium leprae, an obligate intracellular bacterium. Diagnosis of leprosy often relies on skin examinations for clinical signs, bacilli staining from skin smears and invasive s ...
PUBLIC LIBRARY SCIENCE2022Smart pooling: AI-powered COVID-19 informative group testing
Massive molecular testing for COVID-19 has been pointed out as fundamental to moderate the spread of the pandemic. Pooling methods can enhance testing efficiency, but they are viable only at low incidences of the disease. We propose Smart Pooling, a machin ...
NATURE PORTFOLIO2022Enrichment of gut microbiome strains for cultivation-free genome sequencing using droplet microfluidics
We report a droplet microfluidic method to target and sort individual cells directly from complex microbiome samples and to prepare these cells for bulk whole-genome sequencing without cultivation. We characterize this approach by recovering bacteria spike ...
2022Allosteric control of type I-A CRISPR-Cas3 complexes and establishment as effective nucleic acid detection and human genome editing tools
Henning Paul-Julius Stahlberg, Dongchun Ni
Type I CRISPR-Cas systems typically rely on a two-step process to degrade DNA. First, an RNA-guided complex named Cascade identifies the complementary DNA target. The helicase-nuclease fusion enzyme Cas3 is then recruited in trans for processive DNA degrad ...
CELL PRESS2022Nonspecific Binding—Fundamental Concepts and Consequences for Biosensing Applications
Nature achieves differentiation of specific and nonspecific binding in molecular interactions through precise control of biomolecules in space and time. Artificial systems such as biosensors that rely on distinguishing specific molecular binding events in ...
2021Directed evolution of DNA-wrapped single-walled carbon nanotube complexes for optical sensing
Benjamin Paul Johanès Gabriel Lambert
Single-walled carbon nanotubes (SWCNTs) have garnered significant interest for their applications in optical biosensing. SWCNTs exhibit a sensitive and photostable fluorescence in the near-infrared (NIR) range, allowing for the continuous monitoring of tar ...
EPFL2021Organohalide respiration: breakthrough towards the elucidation of the electron-accepting moiety of the respiratory chain
Christof Holliger, Julien Maillard, Adrian Schmid, Lorenzo Cimmino
Background Organohalide respiration (OHR) is a bacterial anaerobic process in which chlorinated compound, e.g. tetrachloroethene (PCE), is used as terminal electron acceptors. Dehalobacter restrictus PER-K23, a paradigmatic organohalide-respiring bacterium ...
2021