Concept

Optical sectioning

Publications associées (161)

Second harmonic generation from nanocrystals under linearly and circularly polarized excitations

Demetri Psaltis, Ye Pu, Rachel Grange, Chia-Lung Hsieh

We study second harmonic generation (SHG) from noncentrosymmetric nanocrystals under linearly polarized (LP) and circularly polarized (CP) excitations. Theoretical models are developed for SHG from nanocrystals under both plane-wave and focused excitations ...

2010

Three-dimensional force measurements in optical tweezers formed with high-NA micromirrors

László Forró, René Salathé, Fabrice Merenda, Sylvia Jeney, Mathieu Grossenbacher

The three-dimensional trap stiffness of optical tweezers formed with high-NA micromirrors is investigated by back-focal-plane interferometry and power spectrum analysis. Normalized stiffness values of kappa(xy)/P-trap =1.2(mu N/m)/mW and kappa(z)/P-trap = ...

Optical Society of America2009

Optical sampling technique applied to high resolution distributed fibre sensors

Luc Thévenaz, Stella Foaleng Mafang

A technique developed to acquire fast optical signals using low frequency detection and acquisition is presented here. It is based on optical sampling that creates a replica of the fast signal on a much slower time scale by a simple strobe effect. High ban ...

SPIE2009

Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy

Pierre Magistretti, Christian Depeursinge, Tristan Colomb, Benjamin Rappaz, Viesturs Simanis, Pierre Marquet, Jonas Kühn, Elena Cano

Digital holography microscopy (DHM) is an optical technique which provides phase images yielding quantitative information about cell structure and cellular dynamics. Furthermore, the quantitative phase images allow the derivation of other parameters, inclu ...

2009

Optical tomography by digital holographic microscopy

Christian Depeursinge, Nicolas Pavillon, Jonas Kühn, Florian Charrière

Optical tomography provides three-dimensional data of the measured specimen, while quantitative phase imaging enables measuring the induced phase-shifts. Combining those two technologies makes possible to get three-dimensional refractive index reconstructi ...

SPIE2009

Optical tomography with digital holographic microscopy

Christian Depeursinge, Nicolas Pavillon, Florian Charrière

Three-dimensional optical imaging by tomographic techniques is presented, especially with object rotation in a transmission configuration. In digital holographic microscopy, the complex field is measured, which permits either to study transparent objects w ...

EOS2009

Determination of local refractive index variations in thin films by heterodyne interferometric scanning near-field optical microscopy

Patrik Willi Hoffmann, Ali Dabirian, Yury Kuzminykh, Giacomo Benvenuti, Maria-Pilar Bernal

We report on a heterodyne interferometric scanning near-field optical microscope developed for characterizing, at the nanometric scale, refractive index variations in thin films. An optical lateral resolution of 80 nm (lambda/19) and a precision smaller th ...

American Institute of Physics2009

Three-dimensional Nano-Localization of Fluroescent Emitters

Theo Lasser, Iwan Märki, François Aguet, Noelia Laura Bocchio

The use of fluorescence light for far-field imaging at the nanometer scale has drawn much attention worldwide. Example techniques include STED microscopy and localization microscopy (PALM, STORM). Typically, nanoscopy approaches have been focused on the ma ...

2009

Model-Based 2.5-D Deconvolution for Extended Depth of Field in Brightfield Microscopy

Michaël Unser, Dimitri Nestor Alice Van De Ville, François Aguet

Due to the limited depth of field of brightfield microscopes, it is usually impossible to image thick specimens entirely in focus. By optically sectioning the specimen, the in-focus information at the specimen's surface can be acquired over a range of imag ...

IEEE2008

Advantages of digital holographic microscopy for real-time full field absolute phase imaging

Christian Depeursinge, Tristan Colomb, Pierre Marquet, Jonas Kühn, Florian Charrière

Different interferometric techniques were developed last decade to obtain full field, quantitative, and absolute phase imaging, such as phase-shifting, Fourier phase microscopy, Hilbert phase microscopy or digital holographic microscopy (DHM). Although, th ...

SPIE2008

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