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Sonde fluorescente

Publications associées (325)

Bloch surface waves-controlled emission of organic dyes grafted on a one-dimensional photonic crystal

Vincent Paeder, Valeria Musi, Francesco Michelotti

An alternative route to plasmon-controlled fluorescence for improving the detection of fluorescence is proposed. In place of a metallic layer, a suitable silicon-based one-dimensional photonic crystal is used to generate a Bloch surface waves-coupled emiss ...

American Institute of Physics2011

Semisynthetic Fluorescent Sensor Proteins Based on Self-Labeling Protein Tags

Matthias Albert Brun

Genetically encoded fluorescent sensor proteins offer the possibility to probe the concentration of key metabolites in living cells. The approaches currently used to generate such fluorescent sensor proteins lack generality, as they require a protein that ...

EPFL2011

Diffraction-unlimited all-optical imaging and writing with a photochromic GFP

Marcel Leutenegger

Lens-based optical microscopy failed to discern fluorescent features closer than 200 nm for decades, but the recent breaking of the diffraction resolution barrier by sequentially switching the fluorescence capability of adjacent features on and off is maki ...

2011

TubeSpin bioreactor 50 for the high-density cultivation of Sf-9 insect cells in suspension

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Xiaowei Zhang, Qiuling Xie

Here we present the TubeSpin bioreactor 50 (TubeSpins) as a simple and disposable culture system for Sf-9 insect cells in suspension. Sf-9 cells had substantially better growth in TubeSpins than in spinner flasks. After inoculation with 106 cells/ml, maxim ...

Springer Netherlands2011

Dynamic measurement of the height and volume of migrating cells by a novel fluorescence microscopy technique

Jean-Jacques Meister, Chiara Gabella, Lara Buscemi Estefanell, Benoît Vianay

We propose a new technique to measure the volume of adherent migrating cells. The method is based on a negative staining where a fluorescent, non-cell-permeant dye is added to the extracellular medium. The specimen is observed with a conventional fluoresce ...

2011

In Vivo Studies on the Control of Gene Expression and Protein Degradation

Karolina Bojkowska

Cellular homeostasis is maintained by tightly regulated gene networks, controlled notably at the levels of transcription, mRNA processing, and protein post-translational modification and turnover. This thesis focuses on two of these regulatory steps, namel ...

EPFL2011

Seeing protein monolayers with naked eye through plasmonic Fano resonances

Hatice Altug, Arif Engin Cetin, Min Huang

We introduce an ultrasensitive label-free detection technique based on asymmetric Fano resonances in plasmonic nanoholes with far reaching implications for point-of-care diagnostics. By exploiting extraordinary light transmission phenomena through high-qua ...

2011

Recombinant expression and functional characterization of the mouse olfactory receptor mOR256-17 in mammalian cells

Horst Vogel, Horst Pick, Luigino Grasso, Thamani Dahoun

Olfactory receptors (ORs) constitute the largest family of sensory membrane proteins in mammals. They play a key role within the olfactory system in recognizing and discriminating a nearly unlimited number of structurally diverse odorous molecules. The mol ...

2011

Sparsity-Driven Reconstruction for FDOT With Anatomical Priors

Michaël Unser, Jean-Charles Baritaux, Kai Hassler, Sebanti Sanyal, Martina Bucher

In this paper we propose a method based on (2, 1)-mixed-norm penalization for incorporating a structural prior in FDOT image reconstruction. The effect of (2, 1)-mixed-norm penalization is twofold: first, a sparsifying effect which isolates few anatomical ...

IEEE2011

A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching

Marcel Leutenegger

Photoswitchable fluorescent proteins have enabled new approaches for imaging cells, but their utility has been limited either because they cannot be switched repeatedly or because the wavelengths for switching and fluorescence imaging are strictly coupled. ...

2011

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