Concept

Sonde fluorescente

Publications associées (325)

Cell Morphology and Intracellular Ionic Homeostasis explored with a Multimodal Approach combining Epifluorescence and Digital Holographic Microscopy

Pierre Magistretti, Christian Depeursinge, Nicolas Pavillon, Pierre Marquet, Jonas Kühn, Pascal Jourdain, Daniel Boss, Aleksandr Benke, Corinne Moratal

The authors have developed a live-cell multimodality microscope combining epifluorescence with digital holographic microscopy; it has been implemented with a decoupling procedure allowing to separately measure from the quantitative phase important cell par ...

2010

Fast molecular tracking maps nanoscale dynamics of plasma membrane lipids

Marcel Leutenegger

We describe an optical method capable of tracking a single fluorescent molecule with a flexible choice of high spatial accuracy (~10–20 nm standard deviation or ~20–40 nm full-width-at-half-maximum) and temporal resolution (< 1 ms). The fluorescence signal ...

National Academy of Sciences2010

Fluorescence Sensors for Lithium Ions and Small Peptides

Kay Severin, Sébastien Rochat

Simple but powerful chemosensors were developed by taking advantage of self-assembly processes. First, we will describe a turn-on fluorescent sensor for the pharmacologically important lithium ion. The sensor can be used in purely aqueous solution, and it ...

Schweizerische Chemische Gesellschaft2010

Three-dimensional nano-localization of single fluorescent emitters

Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler, Noelia Laura Bocchio

We present a combination of self-interference microscopy with lateral super-resolution microscopy and introduce a novel approach for localizing a single nano-emitter to within a few nanometers in all three dimensions over a large axial range. We demonstrat ...

Optical Society of America2010

Transient State Monitoring by Total Internal Reflection Fluorescence Microscopy

Theo Lasser, Thiemo Spielmann

Triplet, photo-oxidized and other photoinduced, long-lived states of fluorophores are sensitive to the local environment and thus attractive for microenvironmental imaging purposes. In this work, we introduce an approach where these states are monitored in ...

2010

Polylactide Nanoparticles Containing Stably Incorporated Cyanine Dyes for in vitro and in vivo Imaging Applications

Li Tang

Stably incorporating fluorescent molecules to polymeric nanoparticles (NPs) or micelles can facilitate the prolonged tracking of these drug-delivery vehicles in vitro and in vivo. However, incorporation of fluorescent molecules, usually charged and thereby ...

Wiley-Blackwell2010

Microfluidic devices made of UV-curable glue (NOA81) for fluorescence detection based applications

Nico de Rooij, Philip Wägli, Blaise Guélat

The UV-curable adhesive NOA81 (Norland Optical Adhesive) is a promising liquid photopolymer for low-cost microfluidic chip production. Different fabrication methods for NOA81 are suggested [1,2]. However, optical characterization for fluorescent based dete ...

2010

Fluorescent nanopigments: Quantitative assessment of their quantum yield

Libero Zuppiroli, Rolando Ferrini

In the last few years, an intense research effort has focused on the synthesis of fluorescent nanopigments for functional inks, light harvesting, tagging, tracing, (bio)labeling, imaging, and lighting applications. Moreover, combined with dielectric matric ...

American Institute of Physics2010

Turn-Off-and-On: Chemosensing Ensembles for Sensing Chloride in Water by Fluorescence Spectroscopy

Kay Severin, Kurt Schenk

The recognition and sensing of aqueous chloride by synthetic receptors is a challenging task. Herein we apply the chemosensing ensemble methodology to optically detect chloride in water at near physiological pH. Variants based on two closely related recept ...

2010

Radiative Engineering of NanoAntenna Arrays for Ultra-Sensitive Vibrational Spectroscopy of Proteins

Hatice Altug, Shyamsunder Erramilli, Mi Kyung Hong

Infrared absorption spectroscopy offers direct access to the vibrational signatures of molecular structure. Although absorption cross sections are nearly 10 orders of magnitude larger than the Raman cross sections, they are small in comparison with those o ...

SPIE-INT SOC OPTICAL ENGINEERING2010

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