Concept

Aequorin

Publications associées (12)

Hyperthermic intrathoracic chemotherapy to control malignant pleural mesothelioma

OBJECTIVE: Malignant pleural mesothelioma (MPM) is a rare disease that has a poor response to conventional therapy. Hyperthermic intrathoracic chemotherapy (HITOC), a treatment combining fever-range hyperthermia with intrapleural cisplatin chemotherapy, ha ...

EPFL2023

Hemoglobin-stabilized gold nanoclusters displaying oxygen transport ability, self-antioxidation, auto-fluorescence properties and long-term storage potential

The development of hemoglobin (Hb)-based oxygen carriers (HBOCs) holds a lot of potential to overcome important drawbacks of donor blood such as a short shelf life or the potential risk of infection. However, a crucial limitation of current HBOCs is the au ...

ROYAL SOC CHEMISTRY2023

A biosensor for measuring NAD(+) levels at the point of care

Johan Auwerx, Kai Johnsson, Luc Patiny, Elena Katsyuba, Qiuliyang Yu, Lin Xue

The cellular level of nicotinamide adenine dinucleotide (NAD(+)), through its different functions, affects cellular metabolism and signalling(1-3). A decrease in the NAD(+) content has been associated with various pathologies and physiological aging(4,5), ...

NATURE PUBLISHING GROUP2019

The Split Luciferin Reaction

Aurélien Godinat

Studying biological processes on the level of live cells with the help of biocompatible reac-tions has tremendously advanced our understanding of basic biology. However, the great complexity of many human pathologies such as cancer, diabetes and neurodegen ...

EPFL2017

Encapsulation of Insulin-Secreting Cells Expressing a Genetically Encoded Fluorescent Calcium Indicator for Cell-Based Sensing In Vivo

Andreas Wiederkehr, Umberto De Marchi, Nicolas Bouche, Christophe Boss, Federico Sizzano, Mouna Conrad

The development of cell-based biosensors that give insight into cell and tissue function in vivo is an attractive technology for biomedical research. Here, the development of a cell line expressing a fluorescent calcium sensor for the study of beta-cell fu ...

Wiley2017

A Biocompatible in Vivo Ligation Reaction and Its Application for Noninvasive Bioluminescent Imaging of Protease Activity in Living Mice

Douglas Hanahan, Aurélien Godinat, Gennady Nikitin, Ke Cheng

The discovery of biocompatible reactions had a tremendous impact on chemical biology, allowing the study of numerous biological processes directly in complex systems. However, despite the fact that multiple biocompatible reactions have been developed in th ...

Amer Chemical Soc2013

Bioluminescent sensor proteins for point-of-care therapeutic drug monitoring

Rudolf Griss

To ensure efficacy but avoid toxicity, many drugs require monitoring of their concentrations in the patient’s blood during therapy. Currently, therapeutic drug monitoring (TDM) is done in diagnostic laboratories that need trained personnel and expensive in ...

EPFL2013

Fluorescence based low-cost microbiosensors using simultaneously microfabrication process and enzyme immobilisation

Nico de Rooij, Peter Van der Wal, Sotiria Psoma

A novel one-step microfabrication process of SU-8 films is proposed for developing inexpensive, small size and light-weight optical glucose micro-biosensors. Experimental work was performed in order to investigate whether the widely used in MEMS applicatio ...

2012

A Ca2+ ion selective electrode biosensor in microfluidics to monitor hepatocyte’s activities

Philippe Renaud, Harald Van Lintel, Robert Meissner

Ca2+ plays a crucial role in cell communication. For example, periodical changes in Ca2+ concentration act as cell sig- nals. Intracellular Ca2+ signals have been measured by Ca2+ sensitive fluorescent dye [1]. In this study, extracellular Ca2+ variation w ...

2010

Semisynthetic fluorescent sensor proteins based on self-labeling protein tags

Kai Johnsson, Marlon Hinner, Matthias Albert Brun, Kui Thong Tan

Genetically encoded fluorescent sensor proteins offer the possibility to probe the concentration of key metabolites in living cells. The approaches currently used to generate Such fluorescent sensor proteins lack. generality, as they require a protein that ...

2009

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