Concept

Glycane

Publications associées (70)

Oligomerization of the alpha 1a- and alpha 1b-Adrenergic Receptor Subtypes: Potential Implications in Receptor Internalization

Horst Vogel, Susanna Cotecchia, Laura Stanasila, Jean-Baptiste Perez

We combined biophys., biochem., and pharmacol. approaches to investigate the ability of the a1a- and a1b-adrenergic receptor (AR) subtypes to form homo- and hetero-oligomers. Receptors tagged with different epitopes (hemagglutinin and Myc) or fluorescent p ...

2003

Sensitivity of polarized epithelial cells to the pore-forming toxin aerolysin

Françoise Gisou van der Goot Grunberg, Laurence Gouzi Abrami

Aerolysin is one of the major virulence factors produced by Aeromonas hydrophila, a human pathogen that produces deep wound infection and gastroenteritis. The toxin interacts with target mammalian cells by binding to the glycan core of glycosylphosphatidyl ...

2003

Influence of cell-line and process conditions on the glycosylation of recombinant proteins

The final glycosylation pattern of recombinant proteins might be determined by the choice of the host cell, cell culture conditions and the purification. Each step influences glycosylation and improved glycosylation of the product may thus be obtained by c ...

EPFL2002

The glycan core of GPI-anchored proteins modulates aerolysin binidng but is not sufficient: the polypeptide moiety is required for the toxin-receptor interaction

Françoise Gisou van der Goot Grunberg, Laurence Gouzi Abrami

Sensitivity of mammalian cells to the bacterial toxin aerolysin is due to the presence at their surface of glycosylphosphatidyl inositol (GPI)-anchored proteins which act as receptors. Using a panel of mutants that are affected in the GPI biosynthetic path ...

2002

Requirement of N-glycan on GPI-anchored proteins for efficient binding of aerolysin but not Clostridium septicum alpha-toxin

Françoise Gisou van der Goot Grunberg

Aerolysin of the Gram-negative bacterium Aeromonas hydrophila consists of small (SL) and large (LL) lobes. The alpha-toxin of Gram-positive Clostridium septicum has a single lobe homologous to LL. These toxins bind to glycosylphosphatidylinositol (GPI)-anc ...

2002

Oxidation of 4-chlorophenol at boron-doped diamond electrode for wastewater treatment

Christos Comninellis, Pierre-Alain Michaud, Ilaria Duo, Marco Panizza

The electrochem. behavior of synthetic B-doped diamond thin-film electrode (BDD) was studied in acid media contg. 4-chlorophenol (4-CP) by cyclic voltammetry, chronoamperometry, and bulk electrolysis. The results showed that in the potential region of supp ...

2001

A specific screen for oligosaccharyltransferase mutations identifies the 9 kDa OST5 protein required for optimal activity in vivo and in vitro

Romain Zufferey

The central reaction in the process of N-linked protein glycosylation in eukaryotic cells, the transfer of the oligosaccharide Glc(3)Man(9)GlcNAc(2) from the lipid dolicholpyrophosphate to selected asparagine residues, is catalyzed by the oligosaccharyltra ...

1997

Identification and characterization of glima 38, a glycosylated islet cell membrane antigen, which together with GAD65 and IA2 marks the early phases of autoimmune response in type 1 diabetes

Steinunn Baekkeskov Hanahan

Immunoprecipitating IgG autoantibodies to glutamic acid decarboxylase, GAD65, and/or a tyrosine phosphatase, IA2, are present in the majority of individuals experiencing pan- creatic cell destruction and development of type 1 diabe- tes. Here we identify a ...

American Society for Clinical Investigation1996

STT3, a highly conserved protein required for yeast oligosaccharyl transferase activity in vivo

Romain Zufferey

N-linked glycosylation is a ubiquitous protein modification, and is essential for viability in eukaryotic cells. A lipid-linked core-oligosaccharide is assembled at the membrane of the endoplasmic reticulum and transferred to selected asparagine residues o ...

1995

Purification and partial characterization of rat factor D

Gerardo Turcatti

Rat factor D has been purified to homogeneity (10559-fold) from serum by chromatography on CM-Sepharose Fast Flow, phenyl-Sepharose CL-4B and Mono S and has been shown to resemble its human and mouse counterparts both in substrate specificity and in its su ...

1991

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