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Publications associées (99)

Examining reactivity and specificity of cytochrome c peroxidase by using combinatorial mutagenesis

Kai Johnsson, Claudio Arrivoli, Petra Tafelmeyer, Martin Wilming

Combinatorial mutagenesis was used to investigate the role of three key residues in cytochrome c peroxidase (CCP) from Saccharomyces cerevisiae, Arg48, Trp51, and Trp191, in control of the reactivity and selectivity of the heme-contg. enzyme. Libraries wer ...

2002

Immunoaffinity screening with capillary electrochromatography

Horst Vogel, Michael Mayer, Markus Ehrat

Highly efficient capillary electrochromatog. sepns. of cardiac glycosides and other steroids are presented. Employing butyl-derivatized silica particles as stationary phase resulted in a nearly three times faster electroosmotic flow (EOF) compared to capil ...

2002

Micromachined impedance spectroscopy flow cytometer for cell analysis and particle sizing

Philippe Renaud, Shady Didier Gawad

A new cytological tool, based on the micro Coulter particle counter (CPC) principle, aimed at diagnostic applications for cell counting and separation in haematology, oncology or toxicology is described. The device measures the spectral impedance of indivi ...

2001

Directed molecular evolution of cytochrome c peroxidase

Kai Johnsson, Petra Tafelmeyer

Cytochrome c peroxidase (CCP) from Saccharomyces cerevisiae was subjected to directed mol. evolution to generate mutants with increased activity against the classical peroxidase substrate, guaiacol, thus changing the substrate specificity of CCP from the p ...

2000

3D optical diffraction tomography in thick highly scattering media using diffuse photon density waves

Near InfraRed (NIR) frequency-domain techniques are promising clinical imaging tools, due to their ability in providing metabolic information about healthy and cancerous abnormalities (tumours, cysts, haematomas, etc.) embedded in thick biological highly d ...

EPFL2000

Fluorescence techniques for fundamental and applied studies of membrane protein receptors: the 5-HT3 serotonin receptor

Horst Vogel, Rudolf Hovius

A fluorescently labeled ligand for the 5-HT3 serotonin receptor was synthesized and its sub-nanomolar affinity for the purified, detergent solubilized receptor was measured. The change in the ligand's fluorescence upon receptor binding was used to directly ...

1999

Treatment of gastrointestinal disease with ppar modulators

Johan Auwerx

Methods and compositions are provided for treating a host having a gastrointestinal (GI) disease by administering to the host a composition containing a pharmaceutically effective amount of a modulator of a Peroxisome Proliferator Activated Receptor (PPAR) ...

1998

Fluorescence photodetection of urothelial neoplastic foci in superficial bladder cancer

Hubert van den Bergh, Georges Wagnières

The prognosis of superficial bladder cancer in terms of recurrence and disease progression is related to the bladder tumor multiplicity and the presence of concomitant 'plane' tumors such as high grade dysplasia and carcinoma in situ (CIS). This study on 3 ...

1997

Evidence for screening effects on the 1/f current noise in GaAs/AlGaAs modulation doped field effect transistors

Marcel Py

Using a delta-doped GaAs/AlGaAs heterostructure with a 10 nm spacer layer, we exploit the metastable nature of the DX centers at low temperatures to control electrostatically their net frozen charge density. The concentration of DX(-) centers at 77 K is on ...

American Institute of Physics1996

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